Teorical Principle; Description - Zuzi UV-VIS 4481/1 Manual De Instrucciones

2. TEORICAL PRINCIPLE

The light absorption of a substance is selective. As a substance is excited by the radiation of light, the
light absorption effect will occur in the substance. Each substance has its own special absorption spec-
trum. When a homogeneous light passes through a solution, the light energy will be lessened, because
some of the energy is absorbed by the solution. The loss of energy is in proportion to the concentration
of substance.
The spectrophotometer 4481/1 can perform chemical quantitative analysis on sample substance accor-
ding to the principle of colorimetry. Within the range of concentration, all parameters are in compliance
with the Beer's law:
Where:
A: Absorbance
T: Transmittance
I: Intensity of transmitting light
I : Intensity of the incident ray
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K: Absorption coefficient of sample
C: Concentration of the substance for measurement
L: Length of sample in optical path

3. DESCRIPTION

Exterior view
Touch screen
The touch screen has two working areas (see picture).
On the right, it's the main menu of functions, and on the left
is the setting of each function.
Manual de instrucciones 54481001
A = log1/T = KCL T = I/I
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LCD screen
Sample door
Version 1 December 2015