Use Of The Microscope In Polarized Light; Centering Of Rotatable Stage - Optika Italy B-510 Serie Manual De Instrucciones

12. Use of the microscope in polarized light

• The system allows observation in Orthoscopy (crossed Nicol) or in Conoscopy (crossed Nicol with
the use of the Bertrand lens).
• For optimal performance in polarized light microscopy, accurate optical adjustments are essential
before beginning the observation.
12.1

Centering of rotatable stage

1. Loosen the stage rotation locking screw ① and
rotate the stage until the graduated scale of the
stage ② and the vernier scale are aligned ③ in
the "0" position. (Fig. 29)
• This operation serves to ensure a standard
reference position for centering the rotating
stage.
2. Focus on a recognizable detail ④ in the field of
view by placing it at the center of the eyepiece
crosshair. (Fig. 30)
3. By turning the stage, the detail in focus will draw
a circle ⑤. (Fig. 30)
4. Move again the stage on the "0" position and lock
the locking screw ①. (Fig. 29)
5. Using the centering screws of the stage ⑥ move
the detail in diametrically opposite direction to the
described circle. The movement must be about
half the diameter of the circle drawn. (Fig. 31)
6. Manually move the specimen and bring it back
to the center of the crosshair. Loosen the stage
locking screw again and rotate the stage again.
7. If the centering has been carried out correctly, by
turning the stage, the image of the focused detail
does not move with respect to the center of the
crosshair. If not, repeat the operations described
by (1) to (6) until the perfect coincidence of the
center of rotation of the stage with the center of
the crosshair for which the specimen remains in
the center of the crosshair when turning the stage.
②
①
⑥
Page 22
③
④
⑤
F ig. 29 F ig. 29
F ig. 30 F ig. 30
⑥
F ig. 31 F ig. 31