Waters HPLC Diagnóstico Y Corrección De Anomalías página 106

Example of Transfer of a Example of Transfer of a
Gradient Method Gradient Method
•1 mL/min
•1.00
•0.80
•0.60 •0.60
•AU
•0.40
•Vo
•0.20
•0.00
•2 •4 •6 •8 •10 •12
Minutes
•14 •16 •18 •20
Change in retention time
Change in resolution
large delay volume
small delay volume
©2007 Waters Corporation 106