Contaminated mobile phase
Contaminated mobile phase
•(b)
•(a)
•Blank gradients 5-80% ACN after
10min (a) and 30min (b) equilibration
w. A eluent =10mM phosphate
buffer pH 7.0
•4 different buffer as A solvent
•Blank gradients 5-80% ACN after
30min (a) and 30min (b) equilibration
w. A eluent =4 different 10mM phosphate
buffers pH 7.0
•HPLC grade water
as A solvent
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