Phase Contrast Observation (Optional); Brightfield Observation (Bf); Phase Contrast Observation (Ph) - Optika Italy B-290 Serie Manual De Instrucciones

8.12 Phase contrast observation (optional)

Slider phase contrast condenser (Fig. 33) allows observation in
brightfield and in phase contrast with 10X/20X/40X objectives.
Observation mode
Phase contrast 10X/20X
Phase contrast 40X
8.12.1 Brightfield Observation (BF)
1. Move the condenser slider in the central position to insert the
empty hole.
2. Center the condenser as described in chapter 8.8 and start
working normally.

8.12.2 Phase Contrast Observation (PH)

1. Center the condenser as already described in the chapter
8.8.
2. Move the condenser slider all the way toward left to insert the
phase ring dedicated to 10X/20X objective. (Fig. 34)
3. Insert 10X or 20X objective into the light path.
4. Open aperture diaphragm.
5. Place a specimen on the stage and focus.
Brightfield
Page 20
Slider position
O
10
40
UPPER PART OF
CENTERING
TELESCOPE
F ig. 33 F ig. 33
F ig. 34 F ig. 34
F ig. 35 F ig. 35