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Operation 5
Back to start position
The program is now back to the start position in the SET mode with the program number
flashing. It is possible to go back to this position at any stage during programming in SET
mode by pressing EXIT or STOP. Note that the program is saved automatically when EXIT
is pressed.
Disabling the current check
See Section 5.4 if you want to use this feature.
5.3
Running a program
Connect the leads from the electrophoresis unit (red to red, and black or blue to blue).
Red is positive and black or blue negative. Up to two electrophoresis units can run at the
same voltage at one time. Please remember to double the maximum current and power
conditions if two units are to be run. Voltage will be the same regardless of how many
units are run. The current should also be doubled if two gels are run on the same unit.
Choosing a program
Press SET and select the program you wish to run by pressing / until the value after
the "P" is correct. Confirm with ENTER. (Omit this step if you have just programmed a
method as described in section 5.2.)
Running a program is described schematically in Fig. 4.
Running
Press RUN to start the electrophoresis. The display will show current values for voltage,
current, power and elapsed time or volthours. You can switch between showing the
elapsed time or volthours by / . The limiting parameter will attain the programmed
limit. The unit for that parameter is underlined. A light emitting diode shows when
voltage is applied (HV on). If no current is displayed, please check the electrical
connections to the electrophoresis equipment.
Pausing
You can interrupt the electrophoresis for sample loading and/or changing parameter
values by pressing PAUSE CONTINUE. Voltage and current will no longer be supplied,
the HV on LED goes off, and you may safely load your samples. The display shows
the status of the run when PAUSE CONTINUE was pressed. Switch between time and
integrated voltage by pressing / . When sample loading is complete, press PAUSE
CONTINUE again or RUN and the run will continue from where it was interrupted. When
in the PAUSE mode you can also press SET to make changes in the program. This mode
is called the P-SET mode. When the P-SET mode is entered you can make changes as
described under programming. The P-SET mode is the same as the SET mode apart
from restrictions in setting the breakpoint. Naturally, it is not possible to enter a time or
volthour that is already passed. PressEXIT or PAUSE CONTINUE to go back from P-SET to
PAUSE. Press RUN or PAUSE CONTINUE to proceed with the electrophoresis.
View programmed values
It is also possible to view the programmed values during a run by pressing SET. Note that
no values can be changed here. The display returns automatically to show RUN values
after 5s. Alternatively use EXIT or RUN.
Electrophoresis Power Supply EPS 601 User Manual 18-1130-19 Edition AD
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