Hoefer HE-PLUS Manual Del Usuario página 13

Note: The addition of Ethidium
Bromide to both the gel and
the running buffer will result in
maximum detection levels by
providing high levels of sample
fluorescence with an evenly low
level of background.
3
Make note of the total solution volume so that degree
of evaporation can be determined and corrected for.
4
Heat the agarose slurry in a microwave oven for
90 seconds. Swirl the flask to make sure any
grains sticking to the walls enter into the solution.
Undissolved agarose appears as small "lenses"
floating in the solution. Heat for an additional
30 – 60 seconds. Re-examine the solution and repeat
the heating process until the agarose completely
dissolves.
5
Add deionized water to replace any volume lost
through evaporation during the heating process.
6
Add your detection reagent (i.e. Ethidium Bromide) to
the manufacturers' recommended concentration. Mix
by gently swirling the flask.
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