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nosepiece (4) until it clicks into place at the
lowest magnification (objective 4x).
Note:
Before you change the objective setting, always
move the microscope stage (7) to its lowest
position. This way, you can avoid causing any
damage!
Now insert the smallest
B/C
eyepiece, in this case
the WF16x (1) into the
eyepiece support (3).
How do I observe the specimen?
After you have assembled the microscope with
the adequate illumination and adjusted it cor-
rectly, the following basic rules are to be ob-
served:
Start with a simple observation at the lowest
magnification. This way, it is easier to position
the object in the middle (centering) and make
the image sharp (focusing).
The higher the magnification, the more light
you will require for a good image quality.
Now place the prepared
specimen (14) directly
under the objective on
the microscope stage.
The object should be
located directly over
the illumination (8).
In the next step, take a look through the eye-
piece (1) and carefully turn the focus knob (11)
until the image appears clear and sharp.
If you would like an even higher level of mag-
nification, insert the 20x eyepiece (2) and turn
the objective nosepiece (4) to a higher setting
(10x or 40x).
Important tip:
The highest magnification is not always the
best for every specimen!
Note:
Each time the magnification changes (eyepiece
or objective change), the image sharpness
must be readjusted with the focus knob (11).
When doing this, make sure to be careful. If you
move the microscope stage too quickly, the ob-
jective and the slide could come into contact
and become damaged!
Which light for which specimen?
With this unit, a reflected light and transmitted
light microscope, you can observe transparent,
semi-transparent as well as non-transparent
objects.
The image of the given object of observation is
"transported" through the light. As a result, only
the correct light will allow you to see something!
If you are observing non-transparent (opaque)
objects (e.g. small animals, plant components,
stones, coins, etc.) with this microscope, the
light falls on the object that is being observed.
From there, the light is reflected back and pass-
es through the objective and eyepiece (where it
gets magnified) into the eye. This is reflected
light microscopy.
For transparent objections (e.g. protozoa), on
the other hand, the light shines from below,
through the opening in the microscope stage
and then through the object.
The light travels further through the objective
and eyepiece, where it is also magnified, and fi-
nally goes into the eye. This is transmitted-light
microscopy.
Many microorganisms in water, many plan
components and the smallest animal parts
are already transparent in nature. Others have
to be prepared. We may make them transpar-
ent through a treatment or penetration with the
right materials (media), or by taking the thin-
nest slices from them (by hand or a specimen
slicer), and then examine them. You can read
more about this in the following sections.
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