Introduction; Kit Composition; Guidelines For The Correct Conservation Of The Kit Components; Materials Required But Not Supplied - INGENASA INgene q IBV SYBR Instrucciones De Uso

INTRODUCTION

INgene q IBV SYBR is a real-time PCR kit suited for the detection of RNA of Infectious Bronchitis virus (IBV) in biological
samples in a simple way, with high levels of sensitivity and specificity.
The kit contains all necessary reagents and enzymes in three mixes. When mixed together, they are at the required
concentrations designed to detect (and optionally quantify) IBV, simply by adding the RNA of the problem sample, amplifying
a region of the spike protein (S1) coding gene. In addition, the master mix contains a passive reference fluorochrome, ROX,
allowing the normalisation of the signal avoiding possible quantification errors deriving from pipetting differences. When
using Applied Biosystems' ABI PRISM PCR models in real time, this ROX is necessary to prevent interferences between the
data collected from various emission channels.
The kit includes an IBV positive control for detection. The kit doesn´t include a standard positive control with a known number
of IBV copies (ask our commercial department for standard control). This control allows generating a standard curve which
links the number of pathogen copies with the cycle threshold (Ct) value.
The kit has been tested for every system from Applied Biosystems (StepOne, HT7300, HT7500) and LC480 from Roche. It is
possible to work with other thermocyclers from other commercial houses, but Ct values may experiment changes.

2. KIT COMPOSITION

COMPONENTS (100 reactions)
Mixture A (IBV specific primers)
Amplification buffer (SYBR)
Mixture B (enzyme mixture)
Positive Control A1 – IBV Amplification control

3. GUIDELINES FOR THE CORRECT CONSERVATION OF THE KIT COMPONENTS

On receiving the kit, keep it at -20ºC until use. The components of the kit are stable for 1 year from the manufacturing date
(see expiry date on packaging).

4. MATERIALS REQUIRED BUT NOT SUPPLIED

Dust-free disposable gloves (without talcum powder).
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Microcentrifuge.
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Tube shaker.
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Real-time thermocycler.
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Micropipettes (0.5-1000 μl).
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Sterile pipette tips (with filter).
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Sterile RNAses / DNAses free water.
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INSTRUCTIONS – ENGLISH
Nº VIALS VOLUME/VIAL
2 600 µl
2 600 µl
1 60µl
1 60 µl
(Versión 1410-14) – PAG.8.