En Instruction Manual - Mettler Toledo 405-DPAS-SC-VP Manual De Instrucciones

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Instructions for Type 405-DPAS-SC-VP
pre-pressurized gel-reference pH Electrodes
1
Introduction
The METTLER TOLEDO Type 405-DPAS-SC-VP combination
pH electrode is a low-maintenance, pre-pressurized,
autoclavable electrode with a gel-type reference and
a VarioPin (VP) connector. The reference element incorporates
a  silver-ion trap to prevent silver sulfide contamination of
the reference junction.
2
Preparation
After unpacking, examine the electrode for any mechanical
damage.
METTLER TOLEDO electrodes are factory tested and are
shipped ready-to-use. (To maintain factory pressurization,
the Type 405-DPAS-SC-VP electrodes have a silicone seal
covering the ceramic diaphragm; this must be removed with
the enclosed blade prior to use.) Remove the hydration cap
and remove any electrolyte crystals by rinsing the electrode
with distilled water. Pat dry; do not rub as this may build up
a static charge and lengthen the response time.
Eliminate any air bubbles around the internal element in the
pH-sensitive membrane.
3
Operation
METTLER TOLEDO pH electrodes can be used with most
pH /mV meters. Follow manufacturer's instructions for use.
A two-point calibration is recommended, using fresh buffers
of known pH values which bracket the sample pH; one buffer
should be close to pH 7 to set the zero point. For fast routine
analysis, a standardization using one buffer at a value near
the sample pH can be done.
4
Sterilization
Type 405-DPAS-SC-VP electrodes are steam-sterilizable
in-situ and auto-clavable. Follow operating instructions for
the autoclave. It is not necessary to use a protective cap on
the connector when autoclaving.
Repeated sterilization may cause the electrolyte to discolor
slightly due to the elevated temperatures. This does not affect
the electrode performance.
5
Maintenance
When properly cared for, METTLER TOLEDO electrodes
should provide accurate results over thousands of uses.
Storage: Electrodes should be rinsed thoroughly after use
and stored in 3M KCl or pH 4 buffer solution with added KCl.
DO NOT store the electrode dry or in distilled or tap water.
If the electrode has been stored dry or in water, it must be
reconditioned by soaking in 3M KCl for several hours.
Refilling: Type 405-DPAS-SC-VP gel-reference electrodes
are not refillable.
Protein Contamination: Clean the electrode with pH Electrode
Cleaner for Proteins following instructions on the bottle.
Reactivating pH membrane: Use Reactivating Solution
following directions CAREFULLY.
© 04 / 16 Mettler-Toledo GmbH
53 500 197
405-DPAS-SC-VP pH Electrodes
405-DPAS-SC-VP pH Electrodes
Printed in Switzerland

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