Operation; Care Of Microscope; Installing Objectives; Substage Condenser - Ryf AG Leica CM E Manual De Instrucciones

Interpupillary distance is changed by simply spreading or pushing
eyetubes on the binocular and trinocular bodies.
If you have purchased a standard or custom outfit, you will notice the
objectives are already installed on the nosepiece and the condenser
is in place under the stage. In this case, go to the OPERATION sec-
tion. If you purchased your CM E in components, please continue.

3.2 Installing Objectives

Attach the objectives to the revolving nosepiece holes counterclock-
wise from the lowest magnification to the highest. Always rotate the
nosepiece by using the knurled nosepiece ring.

3.3 Substage Condenser

Adjust the condenser mount to its lowest position, then insert the sub-
stage condenser into the condenser mount underneath the stage.
Tighten the condenser thumbscrew. Each condenser is furnished with
an iris diaphragm which controls the effective numerical aperture of
the objective. To open and close this diaphragm, simply slide the con-
denser control to the right or to the left.

3.4 Filters

The blue filter is factory installed in the underside of the condenser.
For phase contrast applications replace the blue filter with the green
filter. Special care must be used in handling filters. Scratches and fin-
gerprints will result in poor images and lower light levels.

4.0 Operation

CAUTION:As a safety precaution, the power cord has been
grounded to the microscope base. Never use an adapter bet-
ween the power cord and the power source; it will render the
grounding feature ineffective.
CAUTION : The top collector lens of the illumination system in
the base of the microscope may become warm or hot. Avoid
touching the lens directly.
4.1 Always use your microscope on a hard, stable surface.
4.2 Plug the microscope power cord into a suitable grounded electri-
cal outlet. A grounded 3-wire cord is provided.
4.3 Turn on the microscope with the switch in back and rotate the illu-
mination control knob on the bottom left side of the instrument.
4.4 Set the illumination control knob to the lowest setting. The illumi-
nation control allows you to adjust the intensity of light produced
by the illumination system.
3
4.5 Fully open the aperture diaphragm of the condenser by sliding the
control to the extreme left.
4.6 Using the substage condenser focusing knob, raise the conden-
ser to the top of its excursion.
4.7 Place a specimen slide on the stage.
4.8 Rotate the nosepiece to move the 4x objective into working posi-
tion.
4.9 Raise the stage by rotating the coarse adjustment knob to its posi-
tive stop. Using the fine adjustment knob, bring the specimen into
sharp focus. The CM E has been factory adjusted such that stage
focus can be found 1.5 rotations of highest focus adjustment.
4.10 Adjust the eyetubes for interpupillary distance (IPD) and eye
difference, then set the IPD # for both eyepiece tubes to match the
IPD number on the binocular viewing body scale located between
the eyepiece tubes. Both eyepiece tubes are focusable to
compensate for refractive differences of the eyes. To correctly set
the eyetubes, rotate the 40x objective into place and focus on the
specimen. Then rotate the 4x objective into place and focus each
eyetube separately using the focus ring on each eyetube. Do not
use the coarse or fine focus controls on the side of the instrument
at this point.
4.11 Remove an eyepiece and view the back aperture of the
objective. Close the condenser aperture diaphragm and
then, to obtain the full resolving power of the microscope,
reopen until the diaphragm leaves just disappear from view.
Replace the eyepiece. The aperture diaphragm can be
adjusted to enhance contrast and/or increase the depth of
focus.
4.12 When changing objectives, the positions of the aperture diaphragm
must be reset. As magnification increases, the aperture diaphragm
must be opened as required.
5.0 Care of the
Microscope

5.1 General

Keeping all optical components clean is important for maintaining
good optical performance. The microscope should always be cover-
ed with the plastic dust cover provided with the instrument when it is
not in use. If any optical surface becomes badly coated with dust or
dirt, it should be cleaned by blowing it off with a syringe or brushing
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