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IMPORTANT!
Make sure
that the bottom tank does
not overflow.
Typically 45 – 55 Watts constant power will give good
results with passive ventilation. If the fan kit is used
the power can be increased up to 75 Watts. Ensure
that with the buffer system employed the voltage does
not exceed the maximum recommended voltage. The
resistance of the gel changes throughout electropho-
resis when run at constant power so the starting and
finishing voltages will be different.
!
In general the gel temperature should reach
50 – 60 °C. At temperatures above this range there is
the risk of generating smeared bands or damaging the
gel plates.
3.5 Ending the Run
1
Isolate the power supply before removing the connectors.
2
Remove the safety lid by gripping the edges and push-
ing down with your thumbs on the pegs located on the
top of the unit.
3
Disconnect the leads to the upper and lower
buffer chambers.
4
Open the upper buffer tank drainage tube. The buffer
will then flow into the bottom buffer tank.
5
Remove the lower buffer chamber and carefully
discard the buffer to waste.
6
Dismantle the gel and separate the plates with a
strong, thin, broad blade.
•
p9
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