Calibration - Fisher Finnpipette Novus Instrucciones De Uso

All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with distilled
or deionized water. Normally, the pipettes do not need adjustment, but they are constructed to
permit recalibration and adjustment for liquids of different temperature and viscosity.
Device requirements and test conditions
An analytical balance must be used. The scale graduation value of the balance should be
chosen according to the selected test volume of the pipette:
Volume readable
range graduation
under 10 µl 0.00 1mg
10-100 µl 0.01 mg
above 100 µl 0.1 mg
If the uncertainty of measurement of the balance is known, this may be used instead of the
repeatability and linearity.
Test liquid: Water, distilled or deionized, "grade 3" water conforming ISO 3696. Tests are done
in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air between 20°C
to 25°C.
The relative humidity must be above 50%. Especially with volumes under 50µl the air humidity
should be as high as possible to reduce the effect of evaporation loss. Special accessories,
such as the evaporation trap, are recommended.
Calibration Counter
By selecting MENU -> OPTIONS -> CALIBRATE -> COUNTER the number of pipetttings since
last calibration is shown on the display. The counter is reset to zero when calibration is
performed.
Checking the calibration
The pipette is checked with the maximum volume (nominal volume) and with the minimum
volume. A new tip is first pre-wetted 3-5 times and a series of ten pipettings are done with
both volumes. A pipette is always adjusted for delivery (Ex) of the selected volume. Measuring
volumes taken from balance is not allowed. If the calculated results are in the limits (according
ISO8655), the calibration of the pipette is correct.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the accuracy (A) and precision (cv) of both series.
4. Compare the results to the limits in the Table 1.
If the results are in the limits of Table 1, then the calibration of the pipette is correct.
Otherwise the pipette must be adjusted and checked again.
TABLE1: Maximum permissible errors according ISO 8655
Range Channel
1-10 µl 8, 12
5-50 µl 8, 12, 16
30-300 µl 8, 12
Adjustment
Two point calibration
Normally the two-point calibration should be used.
1. Press MENU and select options with SCROLLKEY and accept with OK.
2. Select CALIBRATE and press OK.

Calibration

Precision
repeatability(s)
and linearity
0.001mg
0.02mg
0.2mg
Volume Accuracy
µl µl
10 ±0.24
1 ±0.24
50 ±1.0
5 ±1.0
300 ±8.0
30 ±8.0
10
Uncertainty of
measurement
0.002µl
0.02µl
0.2µl
Precision
% s.d.µl
±2.4 0.16
±24 0.16
±2.0 0.4
±20 0.4
±2.7 3.0
±26.7 3.0
cv%
1.6
16
0.8
8.0
1.0
10.0