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I. Introduction
GB
Trisensor is a rapid test that allows you to simultaneously detect the presence of both ß-lactam, Sulfonamide and
Tetracycline molecules in a milk sample.
II. Summary of the protocol
•
Add 200 µl of milk into one reagent microwell and mix to homogeneity;
•
Incubate 3 min at 40°C;
•
Dip one Dipstick into each microwell;
•
Continue incubating for 3 min at 40°C;
•
Read the colour intensities.
III. Reaction Mechanism
Trisensor is a competitive test involving two receptors and generic monoclonal antibodies in one single opera
amounts of both receptors and antibodies linked to gold particles. The second is a dipstick made up of a set
line for ß-lactam antibiotics (penicillins and cephalosporins) is located below the sulfonamide line while the line
relating to tetracyclines is located above it. When the reagent from the microwell is re-suspended with a milk
sample, both receptors and monoclonal antibodies will bind the corresponding analytes if present during the
vertically on the dipstick and passes through capture zones. When the sample is free of antibiotics, a colour
sample. On the contrary, the presence of antibiotics in the sample will not cause the coloured signal to appear
IV. Composition of kits
Trisensor Milk Kits contain everything needed to perform 96 measurements.
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12 pots each with 1 strip of 8 reagent microwells and 8 dipsticks;
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1 Micropipette of 200 µl;
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« Positive Standard » containing powder to reconstitute "positive standard" raw milk of PenG,
Sulfadimethoxine and Oxytetracycline (See point X for standards reconstitution). For ease of
recognition, the Positive Std is stained by a dye to give it a very pale red colour;
•
« Negative Standard » containing powder to reconstitute "negative standard" raw milk (See point X
for standards reconstitution).The Negative Std is stained by a dye to give it a very pale green
colour;
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One instruction sheet.
V. Additional material needed
•
1 Heatsensor (40°C incubation, refer to the used Heatsensor manual).
•
1 Readsensor (optional, refer to the Readsensor manual).
VI. General Remarks
1.
If instrumental reading is chosen for result interpretation, the Readsensor must be switched on
before the analysis (see Readsensor manual);
2.
At reception, store the kit in a dry place and at a low temperature between 2°C and 8°C. Before
opening, let the plastic pots reach room temperature and avoid exposure of the product to mois
ture and light;
3.
Avoid using clotted milk with Trisensor;
4.
The best temperature to perform the test is 40°C ± 3°C. Use the "Heatsensor" (or alternatively a
water bath). Any other type of incubator is not appropriate to perform the Trisensor assay. (Refer to
the used Heatsensor manual for setting at right temperature and timing);
5.
After the second incubation, read the result within a 10-minutes time-frame. Do not attempts to
interpret the result after 10 minutes;
6.
When drying, the colour intensities of the lines will become sharper;
7.
8.
Empty one pot before opening another pot.
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