Introduction; Manual Use; Application Of Fluorescent Microscope; Principle Of Fluorescent Microscopy - Auxilab HBF001 Manual De Instrucciones

1. INTRODUCTION

a) Manual use
This operation manual is for 158/358 five wave band Epi-fluorescent microscope. To ensure
safety, get the optimal performance and make you fully familiar with the usage of this
microscope, we recommend that you should read this handbook carefully before operation.
b) Application of fluorescent microscope
Five wave band Epi-fluorescent microscope model 158/358 is used in fluorescent microscopy
techniques, including the fluorescence staining and immunofluorescence.
Fluorescence staining method can show the different components inside the cells specially the
two kinds of nucleic acid (DNA and RNA), and it has a stronger specificity, so this technique is
treated as a good cytochemical method.
Inmunofluorescence is a method which is combined with the technicque of immunology and
fluorescent staining; it has immune specificity and the sensitivity of fluorescence methods. It is
widely used in the field of iatrology, patology and auto-inmune disease diagnosis.
c) Principle of fluorescent microscopy
There are many substances in nature that emit longer wavelength light (fluorescent light) when
they are irradiated by shorten wavelength light (UV, purple, blue or green light); this is called
photo-induced fluorescence. After collected specimen is treated with fluorescent staining, it is
excitated with short wavelength light to obtain fluorescence and adapt for the research of
collected specimen.
Fluorescence dye emits light thus appearing color that can be observed in a dark background;
this technicque is very sensitive and can be perceived with only a little of light under the
microscope. This is why fluorescent dye has become a complex marker, it makes the marked
antibody not only have particular activity, but also be seen by naked eye under the fluorescent
microscope. Only a extremely diluted pigment of fluorescent staining is required. As a result, it
provides a widely use for observing live organs and researching the process and micro-structure
functional organs and the process of a certain metabolic change.
In addition, the fluorescent staining operation is very simple, short time, and easy to make
specimen, specially suitable for quick work, such as the rapid diagnosis of infectious diseases.
Figure 1: The principle of excitation fluorescence.
Manual de instrucciones- Instruction´s manual REF/CODE: HBF001
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