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1.
When viewing a specimen that is not transparent or dark in color you may need to increase the amount of light to
resolve certain features or details. This is best done by simply increasing the brightness of the illuminator by
rotating the brightness control on the on/off switch (14) all the way to its highest setting or rotate the illuminator in the clockwise
direction.
2. When viewing with lower power (4x and 10x) objective lenses you will need to lower the condenser lens in order to
spread the light over the larger field of view. To change the position of the condenser, simply rotate the condenser regulator knob
(8) clockwise until the beam of light spreads wide enough to illuminate the entire field of view when viewing.
3.
As you lower the condenser to spread out the light or change to a higher power objective lens, your image will appear dimmer.
Instead of increasing the light intensity of the illuminator (which may "wash out" fine detail of the specimen you are viewing),
open the aperture of the iris diaphragm to let in more light. Opening and closing the diaphragm (with its knurled ring) will give a
relief view of the specimen and allow you to change the depth of field of the specimen being viewed. The iris diaphragm knurled
ring (see Figure 2f) has a scale with an indicator showing what the N.A. of the diaphragm is.
Figure 2f
4.
For low powers (4x and 10x objectives), you should keep the swing condenser (see Figure 2f) in the light path. However, for
higher powers (40x and 100x objectives), you can turn the condenser knob clockwise to move it out of the light path.
5.
Use the condenser adjustment centering screws (see Figure 2f) to put the specimen image in the center of the field of view. When
the shadow around the field of view of the eyepiece is symmetrical, it shows the condenser has been centered correctly. In fact,
you can enlarge the field diaphragm and make the image tangent to the field of view.
6.
You can adjust the field diaphragm to prevent extraneous light from entering the light path and this limits the diameter of the light
beam entering the condenser. When the image of the diaphragm is just on the edge of the field of view, you can obtain the clearest
image.
7.
The iris scale can be used to help obtain the best resolution and contrast. You will try to match as best you can the N.A. of
illumination with the N.A. of the objective being used. Normally you would put the N.A. of the condenser to 80% of the objective
N.A. For example, the 40x objective has an N.A. of 0.65 which would indicate that you would set the scale on the iris diaphragm
to 0.52 (0.65 x 0.80 = 0.52).
Replacing the Illuminator Bulb and Fuses
1.
Make sure the power is off and carefully lay the microscope on one side.
2.
On the bottom of the microscope, locate and unlock (counter clockwise) the knurled knob that holds the bulb compartment.
See figure 1h.
3.
Remove the knurled knob with the bulb and bulb socket. See Figure 2g.
4.
Remove the old bulb from its socket but make sure beforehand that the bulb has cooled down before touching it.
5.
Install the new bulb by pressing the prongs lightly into the socket.
6.
Replace the bulb and its holder back into the base (15) by lining up the tabs and turning clockwise to lock.
7.
Two fuses are located in the back of the microscope (see Figure 2a). They are 15A T3 fuses. If the power does not come on, it
is possible that one or both fuses needs to be replaced. Make sure all power the power is off and then use a flat head
screwdriver to remove the fuses. If they are blown out, replace them with new fuses and then install the fuse box back in place.
Swing Condenser
Iris Scale
Condenser Adjustment Centering Screws
Illuminator
Figure 2g
14
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