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4
.
First, rotate each eyepiece diopter clockwise so that they are all the way down.
5.
Change the distance between the eyepieces by sliding the eyepiece slide plate in or out horizontally. To adjust the slide plate,
grasp the knurled portion on each side of the plate. Do not hold the eyepieces to adjust the interpupillary distance.
6.
With the 4x objective lens focus with one eye using the coarse and fine focus knobs.
7.
Adjust the eyepiece side plate until the whole field of view can be observed through both eyes at the same time without having
to move your head side to side.
8.
Now read the number off of the eyepiece sliding plate scale. This is your interpupillary distance.
9.
Depending on your individual eyes, you may need to make slight adjustments to the right and left eyepieces for the most
comfortable viewing. Move the diopter up or down until you have the specimen slide in sharp and comfortable focus.
10. You have started with the 4x objective (which is the lowest power objective) and work your way up to higher powers.
11. Look through the eyepiece while turning the coarse focus knob (7) until the specimen comes into view. You may
need to adjust the stage knobs (Figure 1c) slightly to center the specimen in the field of view. Warning: When focusing, be
careful not to raise the specimen stage so high that the specimen slide touches the objective lens. Not only can you break
your slide but you may scratch the objective lens.
12. Finally, adjust the fine focus knob (13) until you reach the sharpest focus for your eye.
Tip: When viewing a specimen with the 100x objective lens, you can improve the resolving power by placing a small drop
of immersion oil between the specimen and objective lens. For specimen slides that you prepare yourself, always cover the
specimen with a thin piece of glass and place the oil on the cover glass. Do not put the oil directly on the specimen sample.
The tension of the coarse focus knob can be adjusted to be tighter or looser for your particular needs by moving the tension
ring clockwise or counterclockwise – see Figure 1e.
Adjusting the Focus Stop
Rack & Pinion
Screw
Adjusting the Focus (Figure 1e)
Tension Adjustment
Ring
Figure 1f
7
Limit Stop Knob
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