Optika B-510 Serie Manual De Instrucciones página 64

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3. Uso of the microscope
► B-510FL ONLY
1.
Turn on the power supply for the mercury
bulb and wait 5 minutes for the arc to
stabilize. (Fig.48).
Move the filter selector ④ to one of the
2.
4 available positions until the click stop .
(Fig.49)
3.
The microscope has a 4-position filter
holder slider. The positions 1 and 2 are
empty to house additional filters, position
3 houses a B filter and position 4 a G filter.
FILTER
EXCITATION
CUBE
FILTER
B
475/30 nm
G
530/40 nm
► B-510LD1/LD2 ONLY:
1.
Turn on the fluorescence LED, using the
switch on the back side of the microscope.
Move the filter selector ④ to one of the
2.
available positions until the click stop.
(Fig.49).
3.
LD1 and LD2 models have a 2-position
filter holder slide. In the case of the LD1
model, the slide only houses a B filter,
while in the LD2 model the slide houses
a B and a G filter.
FILTER
EXCITATION
CUBE
FILTER
B
475/30 nm
G
530/40 nm
DICHROIC
EMISSION
MIRROR
FILTER
505 nm
515LP nm
570 nm
590LP nm
DICHROIC
EMISSION
MIRROR
FILTER
505 nm
515LP nm
570 nm
590LP nm
FITC: fluorescent antibodies
Achridine orange: DNA, RNA
Auramine
Rhodamine, TRITC: fluorescent antibodies
Propidium iodide: DNA, RNA
RFP
FITC: fluorescent antibodies
Achridine orange: DNA, RNA
Auramine
Rhodamine, TRITC: fluorescent antibodies
Propidium iodide: DNA, RNA
RFP
Page 64
APPLICATIONS
APPLICATIONS
Fig.48
Fig.49

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