Agilent ZORBAX Serie Guia Do Usuário página 4

Column conditioning
Every column is tested before shipment and shipped
in the test eluent. So, for first use, rinsing with water is
not necessary. If mobile phase additives are used (such
as buffers or ion-pair reagents) it is advisable to do an
intermediate flush with a mobile phase of the correct
composition, but without these additions. Flushing with
10 to 20 column volumes should help in transitioning to your
mobile phase. For shorter chain chemistries (C8, Phenyl, CN,
for example), care should be taken to make sure the column
has been properly equilibrated prior to use. This will ensure
reproducibility and help prevent retention time drifting. When
using formic acid as a mobile phase additive, condition
the column as recommended in the Column Conditioning
Method Conditions for Formic Acid table.
Column Conditioning Method Conditions for Formic Acid
Column Mobile Phase
id (mm)
2.1 95/5 H
CH
3
formic acid
3.0 95/5 H
CH
3
formic acid
4.6 95/5 H
CH
3
formic acid
Note: Conditioning overnight, or up to 24 hours, may be beneficial,
especially for longer columns or acidic analytes
Flow Rate
(mL/min)
O/ 0.1
2
CN + 0.1%
O/ 0.2
2
CN + 0.1%
O/ 0.4
2
CN + 0.1%
Column Time
Temp. (°C) (hrs)
60 4
60 4
60 4
After
Conditioning
Flush and
store in 100%
CH CN
3
Flush and
store in 100%
CH CN
3
Flush and
store in 100%
CH CN
3