Centering The Field Diaphragm; Using The Microscope; Switching Fluorescence Filter Cubes - Optika Italy IM-3 Serie Manual De Instrucciones

12.2 Centering the field diaphragm
Only IM-3F / IM-3FL4
1. Place the specimen on the stage, insert 10x
objective into the light path and focus.
2. Rotate the field diaphragm lever ①, to fully close
the diaphragm. (Fig. 47)
3. Rotate the two centering screws ② to bring the
bright spot in the center of the field of view.
4. Gradually open the diaphragm. The field
diaphragm is centered when the diaphragm
image is symmetrical to the field of view.
5. In normal use, open the diaphragm until it
circoscribes the field of view.
Effects of the field diaphragm
Field diaphragm adjusts the illuminated area to
obtain a high contrast image.
Set the diaphragm according to the objective in
use until it circoscribes the field of view, in order to
eliminate unnecessary light to eyepieces.

12.3 Using the microscope

IM-3F / IM-3FL4
Turn on the power supply for the mercury bulb and
wait 5 minutes for the arc to stabilize.
IM-3LD
Move the main switch in the position "II" to turn on the
fluorescence illuminator.
12.3.1 Switching fluorescence filter cubes
IM-3F / IM-3LD
Move the filter levers (placed on the right or on the
left sides of the microscope) ③ to insert the desired
filterblock (B or G). (Fig. 48)
③
Page 25
①
②
Fig. 47
Fig. 48