General Description; Filling Instructions - CHART CryoCube Manual Del Operador

The CryoCube vapor shipper is ideally suited for the
transportation of cryobiological materials. The liquid
nitrogen is retained in an advanced QWick charge
absorbent material and the cold nitrogen vapor maintains
the cryo storage area
absorbent retains the liquid nitrogen and prevents
accidental spills.
Liquid nitrogen is classified as "Dangerous Goods" by
the USA Department of Transportation (DOT). At least
three regulatory agencies publish dangerous goods
regulations:
DOT - Department of Transportation
IATA - International Air Transportation
Association
ICAO - International Civil Aviation
Organization
If outside the USA, consult with your local regulatory
authorities to comply with all requirements.
Liquid nitrogen is classified under the name "Nitrogen,
refrigerated liquid" and has a UN 1977 code number. The
name classification and UN Code Number must be
indicated on the shipping carton in addition to a "Gas
nonflammable" label. Packing and labeling requirements
can be obtained from the above organizations. Also,
contact your carrier for any variances in the rules as they
may apply specifically to that company and/or the ultimate
destination of the shipment.
Be aware that the regulations undergo periodic revisions.
If you need current information prior to the shipping of
your cryobiological specimen, please contact your air
carrier for the most recent regulations.

GENERAL DESCRIPTION:

The CryoCube vapor shipper dewar is a vacuum
insulated container of aluminum with fiberglass neck
construction providing the highest efficiency possible in
nitrogen vapor storage. Use the container for inert fluids
only. Liquid oxygen is not compatible with this unit and
must not be stored inside the vapor shipper.
These high quality vacuum insulated units are
constructed of durable materials, compatible with the
divergent temperature extremes and broad applications
of cryobiology. The life expectancy of this product is
two (2) years.
Upon receipt of the container, examine both the container
and packaging for any evidence of damage during
shipping. Contact the shipping agent if any damage is
apparent. Watch after the first fill for any signs of vacuum
loss, such as excessive frost or sweating on the outside
jacket. (Some frost near the top just after filling is
normal).
Ref 20930068 Rev B
between-150°C and -190°C. The
A) SAFETY
To avoid injury by frostbite, use extreme care when handling
liquid nitrogen, liquid nitrogen storage or transfer vessels, or
any objects which have come in contact with liquid nitrogen.
• Leave no area of skin exposed.
• Always wear proper safety attire over clothing,
face shield, cryogenic gloves, and cryogenic
apron.
• Never overfill vapor shippers with liquid nitrogen.
Overfilling the tank may cause immediate or
premature vacuum failure to occur.
• Always keep vapor shippers in upright position.
• Do not tightly seal liquid nitrogen container or
prevent nitrogen gas from escaping.
• Use extreme care to prevent spilling and splashing
liquid nitrogen during transfer.
• Immediately remove any clothing or safety attire on
which liquid nitrogen has spilled.
• Get immediate medical attention for any frostbite
injuries due to liquid nitrogen.
• Never ship the container on its side or upside down,
even when empty. This can lead to vacuum failure
and loss of product inside the vapor shipper.
WARNING: The venting of nitrogen vapors will deplete
oxygen in the air, possibly leading to asphyxiation or even
death. Do not store or use container in areas that have poor
ventilation.
Name and address of manufacturer
Authorized representative in the European Community
B) FILLING INSTRUCTIONS
Note: Fill the container with a funnel or transfer line when
possible. Avoid spilling liquid nitrogen over the vacuum cap on
the side of the dewar, as this can shrink the seal and allow air
to leak into the vacuum space, causing vacuum failrure and loss of
product. To ensure maximum performance from your vapor
dewar simply follow the steps below just prior to shipping to
the final destination:
1. Open carton that dewar is in and remove cork/cover/canister
assembly by lifting straight up.
2. Slowly pour liquid nitrogen into the warm, empty container
until the container weight increases by 2 lbs. (0.91 kg). Pour
slowly to prevent liquid nitrogen from bubbling out of tank.
Note: Follow established safety practices and procedures for
transferring LN 2 .
3. Transfer LN2 using cryogenic hose with a phase
separator, pouring container using a funnel, or other
approved filling device.
4. Replace cork/cover/canister assembly and allow unit to stand
for up to 2 hours (which cools and charges the unit).
5. Pour off any non-absorbed liquid nitrogen just prior to
shipment.
6. Weigh unit and record.
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