Dense And Viscous Liquids; Axygen Pipette Tips; Recommendations - Axygen AXYPET Manual De Instrucciones

5 - DENSE AND VISCOUS LIQUIDS

AXYPET specifications of accuracy and precision are
based on pipetting distilled water. Handling of liquids
with physical qualities of density, viscosity and surface
tension which differ substantially from water may need
gravimetrically checked compensation of the volume
setting. Normally the degree of error resulting from heavy
or viscous liquids is negligible if pipetting is done slowly
and carefully. It is most important to give the liquids
some time to react to the change of pressure by holding
the pipette tip in its position for at least 2 sec. after the
aspiration and the blow out stroke.
If in extreme cases, this method of operation does not
result in accurate values, a compensation may be
achieved as follows:
Weigh the liquid pipetted when the AXYPET is set to the
nominal value. Then calculate the set-off from the nominal
value:
Corr: val. = 2 x nom. val. -
- weight of the sample
m
γ
- density of liquid
Check this operation once again and correct if necessary.
Note the corrected value for further pipetting of the same
kind of liquid.

6 - AXYGEN PIPETTE TIPS

AXYGEN tips are made from high performance
polypropylene and their quality guarantees the precision
and accuracy associated with the AXYPET. Strict control
is maintained throughout the manufacturing process to
ensure the highest quality.
The accuracy and precision figures for the AXYPET are
only guaranteed when AXYGEN tips are used. The use of
inferior quality tips will seriously degrade the performance
of the AXYPET.
Tips 10
These tips are used for volumes between 0.1 μl and 10 μl.
They are used with the AP-2 and the AP-10 models.
Tips 200
These tips are used for volumes between 2 μl and 200 μl.
They are used with the AP-20, AP-50, AP-100 and the
AP-200 models.
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m
-
γ
Tips 250
These tips are used for volumes between 50 μl and 250 μl.
They are used with the AP-250 model.
Tips 1,000
These tips are used for volumes between 100 μl and
1,000 μl. They are used with the AP-1000 model.
Tips 5,000
These tips are used for volumes between 1,000 μl and
5,000 μl. They are used with the AP-5000 model.
Tips 10ML
These tips are used for volumes between 1,000 and
10,000 μl. They are used with the AP-10ML model.

7 - RECOMMENDATIONS

Observing the following recommendations will ensure
maximum possible accuracy and precision of liquid
sampling.
• Make sure to operate the AXYPET slowly and smoothly.
• The depth of immersion in the sample liquid should be
the minimum necessary and should remain constant
during aspiration.
• The AXYPET should be held in a vertical position.
• Change the tip when volume setting is changed or
when a different liquid is to be aspirated.
• Change the tip if a droplet remains on the end of the tip
from the previous pipetting operation.
• Each new tip should be pre-rinsed with the liquid to be
pipetted.
• Liquid should never enter the AXYPET shaft. To prevent
this:
- Press and release the pushbutton slowly and smoothly.
- Never turn the pipette upside down.
- Never lay the pipette on its side when there is liquid in
the tip.
• Never force the volume setting beyond its recommended
limits.
• When pipetting liquids with temperatures different from
the ambient temperature, it is recommended to pre-rinse
the tip several times before use.
• Do not pipette liquids with temperatures above 70°C.
• When pipetting acids or corrosive solutions which emit
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