Cleaning And Sterilization; Pipettor Kit And Accessories - VWR Signature EHP Manual Del Usuario

VWR EHP-03-2008 13 9-04-08
Remove the tip ejector. Unscrew the nut, remove
the shaft, piston assembly, seal and O-ring. Rinse
the removed parts with distilled water and dry
thoroughly. Lightly lubricate the inside surfaces of
the seal and the O-ring with the lubricant. Reassem-
ble the pipette in the reverse order.
If you find an increase in the pipetting force, which
could happen after repetitive autoclaving of the pipette:
Remove the tip ejector. Unscrew the shaft nut, and
then remove the shaft, piston assembly, seal and
O-ring. Rinse the removed parts in distilled water
and dry. Lubricate the internal surfaces of the seal
and O-ring with lubricant that has been included with
each pipette. Reassemble the pipette in opposite
order.
Note: All parts of the pipette can be autoclaved (see
Section 10)
The shaft of the 5000 and 10000 models should be
autoclaved without the filter.
If the problem continues after carrying out the above
steps, contact your VWR representative.
Before returning the pipette, please ensure that the
pipette is completely free of any chemical, radioactive or
microbiological contamination which could pose a threat
during transport and repair. As far as it is possible, clean
the pipette.

10 - CLEANING AND STERILIZATION

Cleaning:
External surfaces of the pipetting pushbutton, the ejector
pushbutton, the handgrip, the shaft nut and the adjustment
knob may be cleaned using a cloth dampened in
isopropyl alcohol. Remaining parts removed from the
pipettor during pipettor disassembly may be washed
with distilled water or isopropyl alcohol.
Sterilization:
The pipettors can be sterilized in the autoclave at 121°C
for 20 minutes. After sterilization, the pipettor should be
dried and cooled down to room temperature.
It is recommended:
- to sterilize the pipettors in autoclave with an initial vacuum
and drying cycle.
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- prior to sterilization to unscrew the shaft nut slightly in
the VWR VE/V2-VWR VE/V1000 pipettes, and unscrew
the shaft slightly in the VWR VE/V5000 and VWR
VE/V10000. After autoclaving these parts should be
screwed tight again.
- to set the locking ring in lower (unlocked)position prior
to sterilisation
- to test pipettor calibration every 10 sterilization cycles.
Note: VWR pipettors should be autoclaved with the
filter removed (VWR VE5000/V5000 and VWR VE10000/
V10000).
After autoclaving, check that the shaft nut is tightly
fitted to the pipettor handle.

11 - PIPETTOR KIT AND ACCESSORIES

Pipettor kit:
The pipettors are delivered in the kits including:
• Pipettor
• Instruction manual
• Calibration key
• Ejector regulation spacers (for 2 μl to 1000 μl pipettors)
• Ejector cap (for 2 μl, 10 μl, 5000 μl and 10000 μl pipettors)
• Identification labels
• Filters (for 5000 μl and 10000 μl pipettors)
• Lubricant
Accessories
Model Tip Choice
VWR VE2/V2,
10 μl
VWR VE10/V10
VWR VE20/V20,
VWR VE50/V50,
200 μl
VWR VE100/V100,
VWR VE200/V200
VWR VE250/V250 300 μl
VWR VE1000/V1000 1000 μl
VWR VE5000/V5000 5000 μl
VWR VE10000/V10000 10000 μl
ENGLISH
Cat. No. US Cat. No. EU
53509-130 613-0334
53508-783 613-0241
53509-126 613-0266
53508-918 613-0273
53503-826 613-0226
16466-000 732-0507
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