Necessary Equipment - Millipore Microfil Manual De Uso

Microfil User Guide

Necessary Equipment

To conduct microbial enumeration on a batch of aqueous filterable samples you
will need:
Samples collected in suitable sterile containers
A Microfil funnel for each sample
An S-Pak™ or an EZ-Pak membrane for each filtration
NOTE: Where the samples to be tested either contain solvents incompatible with
the cellulosic membranes or contain some bacteriostatic compounds that
require substantial rinsing of the membrane to prevent growth inhibition,
the use of Durapore
nearest Millipore office for further details.
At least one Microfil stainless steel support autoclaved prior to testing
NOTE: Remove protective cap before autoclaving. The cap is used only during
shipping to hold the frit in place.
A 3-place stainless steel vacuum manifold (except when using the stand alone
Microfil support)
A vacuum supply and filtrate collection flask
Solid or liquid nutrient media
A 47 mm or a 55 mm Petri dish (solid media) or Petri-Pad™ unit (liquid media)
Filter forceps
An incubator
A funnel dispenser (optional)
A flame for sanitizing support surface between samples
®
membrane may be recommended. Please contact your
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