Bresser BIOLUX NV Instrucciones De Uso página 20

Tip:
i
On the mechanical plate (Fig
5, 28) there are two knurled
screws (Figs 5, A+B). They
are used to precisely position
the specimen laterally (Fig 5,
A) and vertically (Fig 5, B).
Look through the eyepiece (Fig. 1, 1/2) and
turn carefully the focusing wheel (Fig. 1, 9)
until you can see a sharp picture.
Now you can get a higher magnification, while
you pull out slowly the Barlow lens (Fig. 6,
3) of the monoculare barrel (Fig. 6, 5). With
the nearly entirely pulled out Barlow lens the
magnification is raised to 2x.
For still higher magnification you can put the
16x eyepiece (Fig. 2, 2) into the objective
revolver (Fig. 1, 7) and set on higher position
(10x / 40x).
Tip:
i
Depending on the preparation
higher magnifications
not always lead to better
pictures.
With changing magnification (eyepiece or
objective lens changes, pulling out of the
Barlow lens) the sharpness of the image must
be newly defined by turning the focusing
wheel (Fig. 1, 9).
20
nOTe:
Please be very careful when
doing this. When you move
the mechanical plate upwards
too fast the objective lens
and the slide can touch and
become damaged.
8. Viewed Object –
condition and preparation
8.1. Condition
With the Barlow lens nearly fully extended
magnification can
transparent and non-transparent specimens
can be examined with this microscope,
which is a direct as well as transmitted light
model. If opaque specimens are examined
- such as small animals, plant parts, tissue,
stone and so on - the light is reflected from
the specimen through the lens and eyepiece,
where it is magnified, to the eye (reflected
light principle, switch position I). If opaque
do
specimens are examined the light from
below goes through the specimen, lens and
eyepiece to the eye and is magnified en
route (direct light principle, switch position
II). Many small water organisms, plant parts
and the finest animal components have
natural transparent characteristics others
need to be prepared accordingly. Preparation
can take many forms such as the soaking in
appropriate substances (medium) to make
the object opaque. Objects can also be finely
sliced (hand cut, micro-cut). The following
sections explain these procedures in detail.
8.2. Creation of thin preparation cuts
Specimens should be sliced as thin as
possible, as stated before. A little wax or
paraffin is needed to achieve the best results.
A candle can be used for the purpose. The
wax is put in a bowl and heated over a flame.
The specimen is then dipped several times in
the liquid wax. The wax is finally allowed to
harden. Use a MicroCut (Fig 2, 24) or knife/
be doubled. Both
scalpel (carefully) to make very thin slices of
the object in its wax casing.
These slices are then laid on a glass slide and
covered with another.
8.3. Creation of an own preparation
Put the object to be observed on a glass
slide and add a drop of distilled water using a
pipette (Fig. 8, 22a) onto the object (Fig. 8).
DANGER!
Be extremely careful when
using the knives/scalpels
or the MicroCut. There is an
increased risk of injury due to
the sharp edges!