Dic Observation; Koehler Dic Transmitted Light - Optika Italy B-1000 Serie Manual De Instrucciones

14. DIC observation

The microscope allows the observation in Differential Interferential Contrast (DIC) with two different methods: Koehler DIC
and Nomarski DIC.
The Koehler DIC method is the simplest both from the point of view of installation and from the point of view of use, while
the Nomarski DIC method provides for a more complex setup.

14.1 Koehler DIC transmitted light

The Koehler DIC observation in transmitted light requires the kit
consisting of the following accessories: Polarizer ①, transmitted
light Analyzer ②, Interferential green filter ③, DIC slider ④. (Fig.
54)
1. Place the polarizer on the lens at the base of the microscope.
2. Remove the dummy slider from the nosepiece and insert the
analyzer into the dummy slider, then insert the ⑤ assembly
into the slot ⑥. (Fig. 55)
3. Remove the specimen from the stage.
4. Rotate the polarizer at the base of the microscope to achieve
maximum darkening of the eyepieces.
5. Once the maximum darkening is achieved, remove the slider
from the nosepiece, remove the analyzer from the dummy
slider and insert it into the DIC prism. Now insert the DIC
slider ⑦ into the slot ⑥. (Fig. 56)
6. Close the condenser aperture diaphragm a little.
7. Put a specimen on the stage and focus.
8. Begin the observation by rotating the DIC slider knob ⑧ to
obtain a three-dimensional sample effect. (Fig. 57)
• For a better effect on the image you can use the green filter
IF550 which must be placed above the polarizer.
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①
②
⑤
⑥
⑦
⑥
⑧
④
F ig. 54 F ig. 54
F ig. 55 F ig. 55
F ig. 56 F ig. 56
F ig. 57 F ig. 57