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disease. J Clin Immunoassay 1983
Jun;6(2):131–39. 5) Hamilton RG, Adkinson NF.
Clinical laboratory methods in allergic disease.
Lab Management 1983 Dec;21(12):37–50. 6)
Hamilton RG, Adkinson NF. Quantitation of
allergen-specific IgE in serum using the
radioallergosorbent test. J Clin Immunoassay
1983 Jun;6(12):147–54. 7) Homberger HA,
Yunginger JW. Laboratory testing in the
diagnosis and management of allergic diseases.
Clin Lab Annual 1983;2:351–88. 8) Kjellman N-
IM, Johansson SGO, Roth A. Serum IgE levels
in healthy children. Clin Allergy 1976;6:51–59. 9)
Mandy FF, Perelmutter L. Laboratory
measurement of total human serum IgE. J Clin
Immunoassay 1983 Jun;6(2):140–46. 10)
Norman PS. The clinical significance of IgE.
Hosp Pract 1975 Aug;10(8):41–9. 11) Yunginger
JW. Clinical significance of IgE. In: Middleton E,
et al, editors. Allergy principles and practice. Vol
2. 3rd ed. St. Louis: C.V. Mosby, 1988:849-60.
12) National Committee for Clinical Laboratory
Standards. Procedures for the collection of
diagnostic blood specimens by venipuncture;
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H3-A4, Wayne, PA: NCCLS, 1998. 13) Bazaral
M, Hamburger RN. Standardization and stability
of immunoglobulin E (IgE). J Allerg Clin Immunol
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Tables and Graphs
Precision (IU/mL)
Within-Run
3
4
Mean
SD
1
50
1.65
2
107
4.78
3
223
9.85
4
600
24.4
IMMULITE 2000 Total IgE (PIL2KIE-18, 2006-12-29)
1
2
Total
5
CV
SD
CV
3.3%
2.54
5.1%
4.5%
7.20
6.7%
4.4%
15.0
6.7%
4.1%
38.3
6.4%
Linearity (IU/mL)
1
Dilution
Observed
1
8 in 8
14
4 in 8
6.4
2 in 8
3.8
1 in 8
1.8
2
8 in 8
21
4 in 8
11
2 in 8
5.4
1 in 8
3.1
3
8 in 8
195
4 in 8
106
2 in 8
47
1 in 8
26
4
8 in 8
267
4 in 8
133
2 in 8
68
1 in 8
34
5
8 in 8
411
4 in 8
207
2 in 8
97
1 in 8
46
6
8 in 8
823
4 in 8
415
2 in 8
225
1 in 8
121
7
8 in 8
838
4 in 8
381
2 in 8
201
1 in 8
107
8
8 in 8
1,050
4 in 8
536
2 in 8
255
1 in 8
134
2
3
Expected
%O/E
—
—
7.0
91%
3.5
109%
1.8
100%
—
—
11
100%
5.3
102%
2.6
119%
—
—
98
108%
49
96%
24
108%
—
—
134
99%
67
102%
33
103%
—
—
206
101%
103
94%
51
90%
—
—
412
101%
206
109%
103
118%
—
—
419
91%
210
96%
105
102%
—
—
525
102%
263
97%
131
102%
4
5
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