VWR High Performance Guia De Inicio Rapido página 9

Aspirate
Press the push-button to the first stop (this

corresponds to the set volume of liquid).
Hold the pipettor vertically and immerse the tip in

the liquid (see immersion depth table, in the Online
User Manual).
Release the push-button slowly and smoothly
(to top position) to aspirate the set volume of liquid.
Wait one second; then withdraw the pipette tip from
the liquid.
You may wipe any drop lets away from the outside
of the tip using a medical wipe, however if you do so
take care to avoid touching the tip's orifice.
/ For the multichannel models, use a reagent reservoir.
Dispense
Place the end of the tip against the inside wall of the

recipient vessel (at an angle of 10° to 40°).
Press the push-button slowly and smoothly to the

first stop.
Wait for at least a second, then press the push-button

to the second stop to expel any residual liquid from
the tip.
Keep the push-button pressed fully down and (while
removing the pipettor) draw the tip along the inside
surface of the vessel.
Release the push-button, smoothly. Eject the tip by

pressing firmly on the tip-ejector button.
/ For the multichannel models, use a reagent reservoir.
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