Orion Diagnostica QuikRead 101 Manual De Instrucciones página 18

● Make sure that the correct magnetic card for the QuikRead CRP test is used. The
magnetic card must not be bent or exposed to liquids or strong magnetic fields.
● Do not splash liquid into the measuring well of the instrument.
6 Sample collection and preparation
6.1 Fingertip whole blood
Prick the finger with a lancet and discard the first drop. Wipe the finger and collect 20 μl of
blood from the second drop in a glass capillary. Perform the test as outlined in Section 7
"Procedure".
Use of capillaries and plungers
The QuikRead CRP kit Cat. No. 134191 contains 20 μl glass capillaries and plungers, which
are recommended for adding the sample. Insert the plunger into the capillary at the end
with the blue stripe. Fill the capillary with sample up to the white stopper. Ensure that there
are no air bubbles in the capillary. Use a soft tissue to wipe excess sample from the outer
surface of the capillary. Make sure the soft tissue does not draw any sample from inside the
capillary. Put the end of the capillary in the buffer and empty the capillary into the buffer by
pressing down the plunger. Make sure the capillary is emptied completely.
6.2 Alternative sample materials
Anticoagulated whole blood: Whole blood collected in a tube containing heparin or EDTA
can be used. Mix the whole blood by inverting the tube. Perform the test as outlined in
Section 7 "Procedure". The sample volume is 20 μl. The 20 μl capillaries are recommended
for adding the sample.
Serum: Take a normal venous blood sample and separate the serum. Perform the test as
outlined in Section "Procedure". Sample volumes of 12 or 20 μl can be used, noting the
effect of sample volume as stated below.
Plasma: Collect whole blood in a tube containing an anticoagulant such as heparin or
EDTA. Separate the plasma from the blood cells as soon as possible to avoid haemolysis.
Mild haemolysis in samples will not affect the test results. Perform the test as outlined in
Section 7 "Procedure". Sample volumes of 12 or 20 μl can be used, noting the effect of
sample volume as stated below.
Sample volume for serum or plasma samples
1) Pipette 12 µl of the plasma or serum sample. The final result appears in the display.
OR
2) Pipette 20 µl of the plasma or serum sample and multiply the result shown in the display
by 0.6.
Sample storage
Sample material
Anticoagulated whole blood
Serum
Plasma
Samples should not be repeatedly frozen and thawed. Allow the samples to reach room
temperature (18...25°C) before testing. Frozen samples must be thawed completely, mixed
thoroughly, brought to room temperature and centrifuged prior to testing.
6.3 Control materials
QuikRead CRP Control: QuikRead CRP Control is ready-to-use. Perform the test as
outlined in Section 7 "Procedure". The sample volume is 20 μl. The 20 μl capillaries are
recommended for adding the sample.
Other commercially available controls: Handle and use according to the instructions for
each control substance. Serum- and plasma-based controls should be used in the same
way as plasma and serum samples, see Section 6.2.
7 Procedure
Materials required but not supplied
QuikRead 101 Instrument
Capillaries (20 μl) 50 pcs
Plungers 50 pcs
QuikRead CRP Control
Fingertip lancets for the
whole blood procedure
A pipette for dispensing
12 μl volumes
Assay procedure
All reagents should reach room temperature (18...25°C) before use. Upon removal from
refrigerated storage, discard the foil pouch of cuvettes and allow to stay at room temperature
before use. It will take 15 minutes for an individual refridgerated prefilled cuvette to reach
room temperature.
The measurement time of the assay depends on the instrument software version. With the
software version 7.0 or newer, the assay reaction time is max. 1 minute. With older software
versions the assay reaction time is max. 2 minutes.
The subheadings correspond to messages displayed by the instrument.
Read the card
Read the magnetic card by pulling it through the reader slot with the magnetic band facing
you.
Ready for use CRP
1) Remove the foil cover from the cuvette. Beware of splashing the
liquid. Condensed buffer on the foil cover has no effect on results.
The test must be performed within two hours from opening the
cuvette.
2) Make sure that the volume is correct by checking that the liquid
surface is between the two lines marked on the cuvette.
Add 20 µl of undiluted whole blood sample to the cuvette, using a capillary. For plasma
and serum sample volumes and the use of capillaries, see Section 6 "Sample collection
and preparation".
3) Close the cuvette tightly with a CRP Reagent Cap. Put the cap properly on the cuvette
to prevent leakage from the cuvette. Do not press down the blue inner part of the cap.
Do not touch the clear flat surfaces in the lower part of the cuvette (the optical part).
Mix by shaking gently (do not turn the cuvette upside down). Wait until the whole blood
sample solution becomes bright red upon haemolysis. Insufficient haemolysis may
cause the error message "Unstable sample. Please run again" to be displayed. If not
measured immediately, the sample may be stored in the buffer for two (2) hours. Keep
the cuvette in an upright position, do not shake. Before measuring the blank, mix again.
4) Put the cuvette in the measurement well of the instrument.
Short-term storage
2...8°C for 3 days
2...8°C for 7 days
2...8°C for 7 days
Cat. No.
06078
67692 Supplied with 134191
67966 Supplied with 134191
68296 Recommended for quality control
For plasma and serum samples (see
Section 6.2)
Long-term storage
Separate plasma and
store below –20°C
Below –20°C
Below –20°C