Phase Contrast Observation (Ph) - Optika Italy B-1000 Serie Manual De Instrucciones

10.3 Phase Contrast observation (PH)
1. Center the condenser as already described at pa-
ragraph 9.12.
• This condenser does not have a swing-out
lens, so the operation described in step 2 is not
necessary.
2. Rotate the condenser turret to insert the "10/20"
position.
• By inserting any phase ring, the aperture
diaphragm opens automatically. This is a de-
sired effect and should not be considered a
defect.
3. Insert 10x objective into the light path.
4. Place a specimen on the stage and focus.
5. Remove one eyepiece and insert the centering
telescope. (Fig. 51)
6. Rotate the upper part of the centering telesco-
pe until the two phase rings (one dark and one
bright) visible in the telescope are in focus. (Fig.
52-54)
7. Using centering screws on the condenser ① (Fig.
51), center the phase rings to make the bright ring
② be concentric to the dark ring ③. (Fig. 53-54)
8. Insert 20x objective (do not rotate the condenser
turret) and check the centering of the two rings.
9. Repeat the same operation with other objectives
to check the ring centering: 40x objective – tur-
ret position "40", 100x objective – turret position
"100".
10. At the end remove the centering telescope, rein-
stall the eyepiece and begin observation.
• With 40x and 100x objectives it may be use-
ful to slightly raise the condenser, to obtain
a better projection of the phase rings. This is
not a defect.
• With the 4X objective, the condenser could
have a dark halo at the periphery of the field
of view. This is not to be considered a defect.
②
③
Page 28
UPPER PART
OF CENTERING
TELESCOPE
Fig. 51
①
Fig. 52
Fig. 53
Fig. 54