Use Of The Microscope In Polarized Light; Centering Of Rotatable Stage - Optika Italy B-1000 Serie Manual De Instrucciones

12. Use of the microscope in polarized light

• The system allows observation in Orthoscopy (crossed Nicol) or in Conoscopy (crossed Nicol with
the use of the Bertrand lens).
• For optimal performance in polarized light microscopy, accurate optical adjustments are essential
before beginning the observation.
12.1

Centering of rotatable stage

1. Loosen the stage rotation locking screw ① and
rotate the stage until the graduated scale of the
stage ② and the vernier scale are aligned ③ in
the "0" position. (Fig. 39)
• This operation serves to ensure a standard
reference position for centering the rotating
stage.
2. Put the 10x objective in the optical path.
3. Move a small recognizable detail in the field of
view ④ using the X-Y movement knobs on the
mechanical stage and place it at the centre of the
eyepiece crosshair. (Fig. 40)
4. Focus on this detail.
5. By turning the stage, the detail in focus will draw
a circle ⑤. (Fig. 40)
6. Move again the stage on the "0" position and lock
the locking screw ①. (Fig. 39)
7. Using the centering screws of the stage ⑥ move
the detail in diametrically opposite direction to the
described circle. The movement must be about
half the diameter of the circle drawn. (Fig. 41)
8. Using the X-Y movement knobs on the mechanical
stage place the detail in the centre of the eyepiece
crosshair. Loosen the stage locking screw again
and rotate the stage again.
9. If the centering has been carried out correctly,
by turning the stage, the image of the focused
detail does not move with respect to the centre
of the crosshair. If not, repeat the operations
described by 1. to 8. until you reached the perfect
coincidence of the centre of rotation of the stage
with the centre of the crosshair, for which the
specimen remains in the centre of the crosshair
when turning the stage.
⑥
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③
①
②
④
⑤
F ig. 39 F ig. 39
F ig. 40 F ig. 40
⑥
F ig. 41 F ig. 41