Limitations Of Use; To Switch On The Assistboy; Aspirating And Dispensing Liquids; Troubleshooting - bioSan Assistboy Manual Del Usuario

3 - LIMITATIONS OF USE

• The Assistboy may not be used for measuring substances, the vapours of which
damage the following plastics: PP , SI, ABS, EPDM, POM.
• The pipette controller may not be used in an environment where explosion risk is present.
• Flammable liquids shall not be measured - in particular substances with flash-point below
0°C (ether, acetone).
• The pipette controller shall not be used for drawing acids with a concentration above 1 mol.
• The pipette controller shall not be used for drawing solutions with a temperature above
50°C.
• The pipette controller may work in temperature range from +10°C to +35°C.
The pipet controller is suitable for general laboratory use only. It must be used only by per-
sonnel who know the health risks associated with the substances that are normally used
with this instrument.

4 - TO SWITCH ON THE ASSISTBOY

The Assistboy pipette controller is switched on by pressing the aspiration button (fig. 1A) or
the dispense button (fig. 1B). The display will show the selected aspiration mode, dispense
mode and the battery level indicator. Examples of the display indications are shown in
(fig. 5). The battery is discharged and requires charging if the indicator displays one "bar"
(fig. 5.3). When the battery is fully charged, the indicator displays three „bars" (fig. 5.1).
The Assistboy switches off automatically if not used for 5 minutes.
The Assistboy can be charged only with the original charger.
The mains voltage shall conform with the specification on the charger.
Charging shall be done in accordance with section 8 of the instruction manual.

5 - ASPIRATING AND DISPENSING LIQUIDS

Attaching a pipette
Before attaching a pipette check whether the pipette is not damaged, has no dents
or sharp edges in the gripping part. Check whether the gripping part is dry.
The pipette shall be gripped as close to the upper end as possible and carefully inserted
into the pipette holder until resistance is noticed (fig. 3A). Do not apply excessive force
so as not to damage thin pipettes and to avoid injury risk. A pipette that has been correctly
attached and sealed in the holder should not tilt to the sides.
After attaching a pipette hold the device in such a way as to keep the pipettor in vertical
position. The shape of the device casing makes it possible to put aside the pipette controller
with an attached pipette. After work is finished it is recommended not to leave the device
with an attached pipette for a longer period, for example overnight or over a weekend.
Do not put aside the pipette controller if there is liquid in the pipette.
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Filling the pipette
Before aspirating is started, set the suction speed switch using the HIGH/LOW switch (fig. 1C):
• HIGH position - fast aspirating
• LOW position - slow aspirating
It is recommended to choose the LOW position for pipettes with a volume up to 5 ml,
and the HIGH position for pipettes with a volume greater than 5 ml. Holding the pipette con-
troller in such a way that the pipette is in vertical position immerse the pipette end in the liq-
uid to be drawn up (fig. 3B), and press the aspiration button gently. The pipette filling speed
depends on how deep the aspirating button has been pressed. The deeper the button
is pressed the faster the liquid is aspirated into the pipette.
It is recommended to draw a slightly greater liquid volume than required (due to meniscus
above the required volume mark), adjusting the aspiration speed, particularly in the final filling
stage, so as not to overfill the pipette.
Setting the volume
After filling the pipette its end shall be dried with absorbent paper, that does not leave impu-
rities, in order to remove the solution rests from the outside surface of the pipette. Then set
the required liquid volume precisely. Pressing the dispense button gently (fig. 3C), dispense
the excessive liquid from the pipette until the meniscus of the liquid aligns exactly with
the required volume mark on the pipette.
Emptying the pipette
Holding the vessel in inclined position, place the pipette end in contact with the vessel wall
and press the dispense button gently (fig. 3C). The dispensing intensity may be adjusted
depending on how deep the dispense button has been pressed. The deeper the button
is pressed the faster the outflow of liquid from the pipette.
The Assistboy has two dispense modes. The dispense mode is selected with the GRAV/
BLOW switch (fig. 1D).
• GRAV position - the dispense is effected in gravity mode, which means that the liquid
flows out of the pipette by its own weight. The outflow speed is adjusted depending
on how deep the dispense button has been pressed.
• BLOW position - the dispense is effected in gravity mode like in the GRAV mode, however,
when the dispense button is pressed to the end position, the pump is started and fast
emptying of the pipette with a blow is effected.
Attention:
During gravimetrical dispensing the pipette is not completely emptied due to the char-
acte ristics of pipettes used with the pipette

6 - TROUBLESHOOTING

If during your work the functioning of the pipette controller is incorrect, check the cause
and remove the fault.
ENGLISH
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