Gel Pouring - lbx ELFH – 400 – 001 Manual De Usuario

Operation instructions
SETTING UP THE HORIZONTAL GEL TANKS
Instructions for fitting the Electrode Cables:
1. Check the position of the lid on the unit. This shows the polarity and the correct orientation
of the cables: black is negative and red is positive.
2. Remove the lid from the unit. Note that if the lid is not removed, fitting the cables may cause
the gold plug to loosen and damage the electrode.
3. Screw the cables into the tapped holes as tightly as possible so that there is no gap between
the lid and the leading edge of the cable fitting.
4. Refit the lid.

GEL POURING

Before pouring, let the gel cool to 50-60°C.
USING THE GEL BOX
1. Place the gel box on a level surface and place a gel tray that fits inside. To prevent
leaking, both ends of the gel tray must be closely against the gel box.
2. Place the comb(s) in the tray.
3. Pour the agarose carefully so as not to make bubbles.
4. Let the gel settle and wait for it to set completely.
5. Pull the comb(s) out carefully and move the tray with the gel into the main tank.
USING THE GEL CASTING TRAY
1. Place the gel casting tray on a level surface and place a gel tray inside. Make sure the
both ends of the tray are closely against the silicone mat.
2. Use the wrench to tighten the gel tray.
3. Put the comb(s) in the tray.
4. Pour the agarose carefully, so as not to generate bubbles. If any bubbles form, they can
be smoothed and dispersed with the tip of a pipette.
5. Let the gel settle and wait for it to set completely.
6. Pull the comb(s) out carefully and move the gel tray to the main tank.