Quality Control - AIDIAN go CRP Manual Del Usuario

6 Sample collection and preparation
Sample material, collection and volume
Sample Sample
material volume
Fingertip whole
blood
Anticoagulated
whole blood
Plasma
Serum
*If you want to use sample volume 12 μl in order to have a wider measurement range, you have
to change the settings of the QuikRead go instrument. Please see Instrument manual: Settings →
Measurement flow → Test parameters.
Sample dilution
Sample material
Whole blood
Plasma/serum
Sample storage
Sample material
Fingertip
whole blood
Anticoagulated
whole blood
Plasma
Serum
Sample (whole blood,
plasma, serum) in buffer
Allow the samples to reach room temperature (18...25ºC) before testing. Frozen samples
must be thawed completely, mixed thoroughly and brought to room temperature prior to
testing. If the thawed plasma samples contain clots the samples shall be centrifuged.
Samples should not be repeatedly frozen and thawed.
7 Procedure
Materials required but not supplied
Material
QuikRead go Instrument
®
Capillaries (20 μl) 50 pcs
Plungers 50 pcs
QuikRead go CRP Control
®
QuikRead go CRP Control High
®
QuikRead CRP Control
®
QuikRead go CRP Control High
®
Fingertip lancets
Assay procedure
Discard the foil pouch of the cuvette rack and mark the opening date on the rack.
Sample collection (Fig. 1–5)
Glass capillaries (20 μl) and plungers are recommended for adding the sample into
a prefilled cuvette. Capillaries (Cat. no. 67962) and plungers (Cat. no. 67966) can be
purchased separately, whereas the QuikRead go CRP kit (Cat. no. 135171) contains them
already.
1
A prefilled cuvette must reach room temperature (18...25ºC) before use. This will take 15
minutes for an individual refrigerated (2...8ºC) prefilled cuvette. Do not touch the clear
flat surfaces in the lower part of the cuvette (the optical part). Remove the foil cover
from the cuvette. Beware of splashing the liquid. Condensed buffer on the foil cover has
no effect on results. The test must be performed within two (2) hours from opening the
cuvette. The liquid surface should lie between the two lines marked on the cuvette.
2 Insert the plunger into the capillary at the end with the blue stripe.
3 Fill the capillary with the sample up to the white stopper. Ensure that there are no air
bubbles in the capillary. Use a soft tissue to wipe away any residual sample from the
outside of the capillary.
4 Place the sample capillary into the buffer solution in the cuvette and dispense by
pressing down the plunger. Make sure that the capillary is completely empty.
5
Close the cuvette tightly with a CRP reagent cap. Do not press down the blue inner part
of the cap. Once the sample is added to the buffer, it may be held for up to two (2) hours
prior to running the assay. Keep the cuvette in an upright position, do not shake.
Analysing the sample (Fig. 6–8)
See the QuikRead go Instrument manual for detailed instructions. The display guides you
in performing the test.
6 Choose Measure on the display of the QuikRead go instrument.
7 Insert the cuvette in the measurement well of the instrument. The barcode should be
facing you as shown in the Figure 7. The instrument recognises the sample type. The
display shows how the measurement is proceeding. First the instrument measures the
sample blank. After that the instrument measures the CRP concentration within one
minute.
8 When the measurement has been completed, the result will be shown in the display and
the cuvette will rise up from the measurement well automatically.
Analysing the control (Fig. 7–8)
Please see the instructions for use provided with each control vial. Analyse control samples
as patient samples but choose Quality Control on the display of the QuikRead go
instrument (Figures 7–8). The result will be stored as the control measurement.
8

Quality control

Regular use of QuikRead go CRP Control (Cat. no. 153764) and/or QuikRead go CRP
Control High (Cat. no. 153763) is recommended. Alternatively QuikRead CRP Control (Cat.
no. 68296) and/or QuikRead go CRP Control High (Cat. no. 137071) can be used for quality
control.
Sample collection
Prick the clean and dry finger with a lancet and discard the first drop.
20 μl Wipe the finger and collect 20 μl of blood from the second drop in
a capillary.
Whole blood collected in a tube containing heparin or EDTA can be
20 μl used. Mix the whole blood by inverting the tube several times and
take 20 µl with a capillary or pipette.
20 μl Whole blood collected in a tube containing heparin or EDTA can be
or used. Avoid haemolysed samples. Mild haemolysis in samples will
12 μl * not affect the test results.
20 μl
Avoid haemolysed samples. Mild haemolysis in samples will not
or
affect the test results.
12 μl *
Instructions
Do not dilute whole blood samples.
Plasma or serum samples may be diluted with a 0.9% NaCl solution before
adding the samples to the cuvettes. Dilution ratio is 1+3 (1 part of volume
sample + 3 parts of volume 0.9% NaCl). Take 20 µl of the diluted sample
into a cuvette, analyse the sample and multiply the result by 4.
Short-term storage
Not more than 15 minutes
in the heparinised capillary
2...8°C for 3 days
2...8°C for 7 days
2...8°C for 7 days
18...25°C for 2 hours
Cat. no.
133893
67962 Supplied with 135171
67966 Supplied with 135171
153764 Recommended for quality control
153763 Recommended for quality control
68296 Alternative quality control
137071 Alternative quality control
Long-term storage
Do not store
Separate plasma and
store below –20°C
Below –20°C
Below –20°C
Do not store