Setting The Volume; Method Of Pipetting - Axygen AXYPET Manual De Instrucciones

2 - SETTING THE VOLUME

Setting of the volume of the aspirated liguid can be done
using either the lower section of the pushbutton (Fig. 1A2)
or by the black knurled adjustment knob (Fig. 1B). The
volume shown by the counter is indicated by three digits,
which should be read from top to bottom. Typical volumeter
readings are shown in the following table:
Counter
Model
readings
0
AP-8-10
3
AP-12-10
5
0
AP-8-50
6
AP-12-50
5
0
AP-8-200
8
AP-12-200
5
2
AP-8-300
5
AP-12-300
0
To set the required volume, use the lower section of the
pipetting pushbutton (Fig. 1A2) or the black adjustment
knob (Fig. 1B), to increase or reduce the volume. All vol-
ume setting procedures should end in the the reduction
of volume setting from a position just above the desired
level to that level. As further examples:
• When moving from a high volume setting to a lower
value, the pushbutton or adjustment knob should be
rotated at normal speed, clockwise, to take the setting
just above the desired level. It should then be further
rotated very slowly till the desired volume is shown on
the digital volume display.
• In the event that you overshoot the desired setting,
repeat the procedure by increasing the volume to
a position just above the desired volume and then once
again, reduce slowly to the desired level.
•When moving from a low volume setting to a higher
value, the pushbutton or adjustment knob should be
rotated at normal speed, anticlockwise, to take the set-
ting to a position just above the desired level. Then
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Set Basic
volume degree
3.5 μl 0.02 μl
6.5 μl 0.10 μl
85.0 μl 0.20 μl
250.0 μl 1.0 μl
reduce the reverse the rotation and slowly adjust back
until the desired volume is shown on the digital display.
• Once again, in the event that you overshoot the desired
setting, repeat the procedure by increasing the volume
to a position just above the desired volume and then
reduce slowly to the desired level.

3 - METHOD OF PIPETTING

Fit the tips onto cones of the multichannel module. To fit
tips on the multichannel shafts, hold the pipette vertically
and press it against the tips in the rack box, until the
shafts retreat about 1.5 mm into the multichannel module.
The suspension system of the shafts ensures even and
tight sealing of tips. A rolling movement does not have to
be performed to seal the tips tightly. The liquid aspirated
into the tips should not leak if the tips are fitted properly.
While holding the pipette in a vertical position, tips
should be immersed in the liquid to a depth of 2-4 mm,
and flushed once by drawing a dose of liquid and
dispensing it with a slow and steady movement. Next,
still holding the pipette vertically, the operator should
press the pipetting button until the first resistance point is
felt, and immerse the tips in the liquid to the depth of 2-4
mm, (Fig. 3B). Releasing the pipetting button with a slow
and uniform movement over 2-3 seconds, the liquid
should be drawn into the tips, (Fig. 3B) which should
then be lifted above the liquid's surface. The pipette
should next be positioned at an angle of 10-45° in relation
to the inner walls of the destination vessels and tips
should be emptied by pressing the pipetting button slowly
until the first resistance point is felt, (Fig. 3C). After waiting
a further second, the pipetting button should be pressed
to the second resistance point in order to expel any
remaining liquid, (Fig. 3D). The tips should then be lifted
out from the vessels while maintaining contact between
the ends of the tips and the inner walls of the
vessel until the pipetting button has been released, (Fig.
3E). Finally, pressing the ejector button, the operator
should separate the tips from multichannel module's
cones, (Fig. 3F).
ENGLISH
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