Cleaning And Sterilisation; Pipette Kit And Accessories - Axygen AXYPET Manual De Instrucciones

water density coefficient [μl/mg], which depends on tem-
perature and pressure according to the following table:
Temperature [°C]
20
21
22
23
24
25
If the average aspirated volume exceeds the permissible
value, the following should be done:
• Remove the pipetting pushbutton, (Fig. 4A),
Warning: The pipetting pushbutton consists of 2
parts: the lower volume setting section (Fig. 1A2) and
the upper button (Fig. 1A1). After removal of the
pushbutton, both parts are separated.
• Holding the volume setting knob to protect it against
rotation, insert the calibration key into the cuts of the
calibration screw, (Fig. 4B),
• Turn the key clockwise to reduce the aspirated volume,
or counter-clockwise to increase the volume. One full
turn of the calibration key changes the pipette aspiration
volume by the amount shown in the table, (Fig. 4C),
• Take out the key and re-fit the pipetting pushbutton
(Fig. 4D). The pipetting pushbutton should be fixed by
placing the lower volume setting section on the arbor
first (Fig. 1A2) and then the upper button (Fig. 1A1).
Determine the average aspirated volume. The average
volume should be within the permissible range given in
the table. If the volume exceeds the values stated, the
recalibration procedure should be repeated.

6 - CLEANING AND STERILISATION

Cleaning
External surfaces of the pipetting pushbutton, the ejector
button, the handgrip, shaft and the adjustment knob may
be cleaned using a cloth dampened in isopropyl alcohol.
Sterilisation:
The pipette can be sterilised in the autoclave at 121°C for
20 minutes. After sterilisation, the pipette should be dried
and cooled to room temperature.
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Pressure [kPa]
95.0 101.3 105.0
1.0028 1.0029 1.0029
1.0030 1.0031 1.0031
1.0032 1.0033 1.0033
1.0034 1.0035 1.0036
1.0037 1.0038 1.0038
1.0039 1.0040 1.0040
It is recommended:
- to sterilise the pipette in autoclave with an initial vacuum
and drying cycle,
- prior to sterilisation unscrew the nut connecting the
handle and module slightly. After autoclaving these
parts should be screwed tight again.
The precision of the results should not alter if the pipetting
process and autoclaving are carried out as described in
this manual. Because a slight change in the accuracy of
the dosage may occur, it is recommended to:
- check the calibration of the pipette after the initial first,
third and fifth autoclaving cycles and then after every
10 autoclaving cycles.

7 - PIPETTE KIT AND ACCESSORIES

Pipette kit:
The pipettes are delivered in the kits including:
• Pipette
• Instruction manual
• Calibration key
• Identification labels
• Right Hand Ejector Button
Accesories:
Axygen
Cat No. Pipet Tips
AXYPET 8
AP-8-10 T-300-R
AP-8-50 TR-222-C-R
AP-8-200 TR-222-C-R
AP-8-300 T-350-C-R
AXYPET 12
AP-12-10 T-300-R
AP-12-50 TR-222-C-R
AP-12-200 TR-222-C-R
AP-12-300 T-350-C-R
ENGLISH
Sterile Filtered
Pipet Tips Pipet Tips
T-300-R-S TF-300-R-S
TR-222-C-R-S TF-50-R-S
TR-222-C-R-S TF-200-R-S
T-350-C-R-S TF-350-R-S
T-300-R-S TF-300-R-S
TR-222-C-R-S TF-50-R-S
TR-222-C-R-S TF-200-R-S
T-350-C-R-S TF-350-R-S
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