Recommendations; Recalibration - Axygen AXYPET Manual De Instrucciones

4 - RECOMMENDATIONS

To achieve maximum safety, precision and reliability, the
following principles should be observed:
• do not draw liquids without tips fitted on the pipette
cones,
• do not lay the pipette down with tips filled,
• do not draw volumes of liquid exceeding the pipette's
volume range,
• check if the tips are fitted properly,
• while operating, the pipette must be held vertically,
tips should be immersed in liquid to the depth of 2 to 4
millimeters and the pipetting button should be depressed
and released slowly and evenly,
• new tips must be re-wetted prior to pipetting, by drawing
and expelling the liquid to be measured. This is especially
important when working with liquids of viscosities and
densities different to that of water and in temperatures
different from ambient,
• when pipetting liquids which tend to wet walls of tips,
such as serums, proteins or organic solvents, measuring
must be performed much slower than with other liquids,
• tips must be replaced with new ones, when changing
from one liquid to another, or if drops of liquid remain
inside the tips,
• after use, the pipette should be stored vertically in a
holder with tips removed.

5 - RECALIBRATION

AXYPETS are calibrated gravimetrically, using AXYGEN
tips and distilled water, at the temperature 20±1°C,
according to EN ISO 8655 standard.
If during pipette operation, you find that the accuracy
error (the difference between the real aspirated volume
and the preset volume) exceeds the permissible value
shown in the table in section 1, the pipette recalibration
procedure should be carried out.
Before starting the recalibration procedure it is necessary
to check whether the following requirements were met
during error determination:
• the ambient temperature, and the temperature of the
pipette, tips and water was identical
• the density of the liquid used is close to that of distilled
water
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• a balance with appropriate sensitivity has been used
Volume checked [μl]
0.1 - 10
10 - 100
> 100
• mg/μl conversion factor has been taken into account
• the requirements given in sections 3 and 4 have been
met
If the above conditions are satisfied and the accuracy
error for selected volume given in section 1 exceeds the
permissible value, the pipette recalibration procedure
should be carried out.
The recalibration can be performed within one full turn
of the key to the right or to the left only.
Recalibration conditions:
• Ambient temperature and the temperature of the
pipette, tips and liquid should be within the range
20-25°C and stabilised during weighing within ±0.5°C
• Measurements should be conducted using distilled
water
• Balance sensitivity should be suitable for the volume to
be measured
Recalibration procedure:
• Set the dose volume depending on the pipette volume
according to the following table:
Range
of the Preset
Model pipette volume
volumes [μl]
[μl]
AP-8-10
1 - 10 1
AP-12-10
AP-8-50
5 - 50 5
AP-12-50
AP-8-200
20 - 200 20
AP-12-200
AP-8-300
50 - 300 50
AP-12-300
• Perform three aspiration series (each series should
include the aspirations from all channels), weigh each
time and calculate the average value of the aspirations.
• Calculate average aspirated volume in μl multiplying
the average aspiration amount [mg] by the distilled
ENGLISH
Balance sensitivity [mg]
≤ 0.001
≤ 0.01
≤ 0.1
Volume change
Permissible Δ Δ V for full turn
volumes of the calibration
[μl] key [μl]
(24 increments
0.92 - 1.08 0.33
4.8 - 5.2 1.67
19.4 - 20.6 6.30
49.2 - 50.8 10.00
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