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- Página 1 Spectrophotometer Genova Nano Instruction Manual 737 555/Rev E/05-17...
- Página 2 Safety Please read this information carefully prior to installing or using this equipment. 1. The unit described in this manual is designed to be operated only by trained personnel. Any adjustments, maintenance and repair must be carried out as defined in this manual, by a person qualified to be aware of the hazards involved.
- Página 3 Merci de lire attentivement ces informations avant d'installer ou d'utiliser cet appareil. 1. L'appareil décrit dans ce manuel est conçu pour être utilisé uniquement par des personnes formées. Tout réglage, maintenance ou réparation doit être effectué comme décrit dans ce manuel, par une personne qualifiée consciente des risques encourus.
- Página 4 Bitte lesen Sie diese Hinweise vor Installation oder Gebrauch dieser Ausrüstung sorgfältig durch. 1. Das in diesem Handbuch beschriebene Gerät darf nur von geschultem Personal bedient werden. Alle Anpassungen, Wartungsarbeiten und Reparaturen müssen entsprechend der Vorgaben in diesem Handbuch und von einer kompetenten Person, die mit den damit verbundenen Gefahren vertraut ist, durchgeführt werden.
- Página 5 Leggere attentamente queste istruzioni prima di installare o utilizzare il dispositivo. 1. L'unità descritta nel presente manuale è stata realizzata per essere utilizzata solo da personale che ha ricevuto l'apposita formazione. Qualsiasi operazione di regolazione, manutenzione e riparazione deve essere effettuata sulla base di quanto indicato nel presente manuale da personale qualificato consapevole dei rischi connessi.
- Página 6 Lea esta información atentamente antes de instalar o utilizar este equipo. 1. La unidad descrita en este manual está diseñada para que solamente la utilice personal con formación. Cualquier operación de ajuste, mantenimiento y reparación debe llevarse a cabo del modo indicado en este manual y debe realizarla una persona cualificada que sea consciente de los peligros que implica.
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Página 7: Tabla De Contenido
Contents SECTION 1 - INTRODUCTION ................9 INSTRUMENT DESCRIPTION..................9 GENOVA NANO WITH MICRO VOLUME ACCESSORY SPECIFICATION ....9 UNPACKING........................10 SECTION 2 – ACCESSORY LAYOUT AND INSTALLATION ......11 ACCESSORY LAYOUT ....................11 INITIALISATION ......................11 Note: The instrument will return to the last main menu used....... 11 SECTION 3 –... - Página 8 SECTION 11 – TROUBLESHOOTING ..............28 11.1 CALIBRATION ERROR CODES ................... 28 11.1.1 Calibration Procedure Error Codes ................28 11.2 TROUBLESHOOTING GUIDE ..................28 11.3 TECHNICAL SUPPORT ....................28 SECTION 12 – GLOSSARY OF ICONS ............. 29 SECTION 13 – CHEMICAL COMPATABILITY ..........30 SECTION 14 –...
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Página 9: Section 1 - Introduction
SECTION 1 - INTRODUCTION INSTRUMENT DESCRIPTION The Genova Nano is a UV/visible spectrophotometer dedicated to life science analysis. This spectrophotometer incorporates a micro volume sample measurement accessory that allows sample volumes as low as 0.5µl to be analysed. In addition to the standard measurement modes: photometrics, concentration, multi-wavelength, spectrum scanning, quantitation and kinetics, the Genova Nano spectrophotometer is pre-programmed with methods to determine DNA concentration and purity ratios using wavelengths recorded at 260, 280 and 230nm, with an optional... -
Página 10: Unpacking
UNPACKING Please check that the following items are included in the packaging: Genova Nano spectrophotometer (737 503) • 4GB USB memory stick (019 146) • Universal power supply 24V, 65W (021 060) • Calibration standards with certificate (035 092) • Genova Nano instruction manual (737 555) •... -
Página 11: Section 2 - Accessory Layout And Installation
SECTION 2 – ACCESSORY LAYOUT AND INSTALLATION ACCESSORY LAYOUT INITIALISATION Connect the power supply unit to the power inlet socket on the rear panel of the instrument and connect to the mains socket. Turn the power on at the mains and switch the instrument on using the power switch on the rear of the instrument. -
Página 12: Section 3 - Micro-Volume Settings
SECTION 3 – MICRO-VOLUME SETTINGS ACCESSING THE MICRO VOLUME SETTINGS The Micro Volume icon is displayed in the bottom right hand corner of the screen in each measurement mode. Micro Volume Icon Fig 3.1 – Accessing the Micro Volume Settings Menu The micro volume settings are accessed by pressing the key below the Micro Volume icon. -
Página 13: Path Length Selection
PATH LENGTH SELECTION 3.2.1 Known Path Length Measurements If the required measurement path length is known it can be selected by pressing the button adjacent to the 0.2mm or 0.5mm Path Length Setting icons. The selected setting is indicated by an icon with a black background. -
Página 14: Section 4 - Performing Micro Volume Measurements
SECTION 4 – PERFORMING MICRO VOLUME MEASUREMENTS For detailed descriptions of the measurement modes that are available on the Genova Nano spectrophotometer please refer to the supplied Genova Plus operating manual. PIPETTING SAMPLES ONTO THE MICRO VOLUME READ HEAD The Genova Nano spectrophotometer is designed to measure sample volumes ranging from 0.5µl to 5.0µl. -
Página 15: Sample Recovery Or Removal
SAMPLE RECOVERY OR REMOVAL Once a measurement is complete, the sample solution can be recovered from the lower read head with a suitable pipette or removed from the read heads by cleaning with a suitable lint free cloth. 1. Open the lid of the spectrophotometer. The read head mechanism will open to allow access to the upper and lower read heads... -
Página 16: Section 5 - Top 10 Tips For Sample Measurement
SECTION 5 – TOP 10 TIPS FOR SAMPLE MEASUREMENT 1. Ensure the read heads are clean. Wipe both the upper and lower read heads with a lint-free cloth wetted with deionised water to remove any residues of previous samples. Dry with a fresh cloth. -
Página 17: Section 6 - Step By Step Guide To Making A Dna Measurement
SECTION 6 – STEP BY STEP GUIDE TO MAKING A DNA MEASUREMENT The DNA measurement mode of the Genova Nano allows the user to select a method from a list of common nucleic acid measurement tests, including single wavelength concentration measurements for dsDNA, ssDNA, RNA and Oligonucleotides and methods that use absorbance ratios for estimating nucleic acid purity, such as 260nm/280nm and 260nm/230nm. -
Página 18: Multi-Wavelength Mode
5. Lift the lid of the Genova Nano and pipette 2µl of water onto the read head. Close the lid. Perform the blank reading. 6. Lift the lid and wipe the water from both the upper and lower read heads. 7. -
Página 19: Section 7 - Calibration Of The Micro Volume Accessory
SECTION 7 – CALIBRATION OF THE MICRO VOLUME ACCESSORY Jenway recommends that the micro volume accessory is calibrated every 6 months. A set of calibration solutions (Part code 035 092) are supplied with the Genova Nano spectrophotometer. Please note that the calibration solutions should be discarded 1 week after being opened. -
Página 20: Calibration And Verification
2. Select the digit to be changed using the keys at the bottom of the screen. Use the keys adjacent to the Arrow icons to increase or decrease the number. Press the key adjacent to the Tick icon to save any changes. 3. - Página 21 During the calibration process, there is the option to log the calibration data to USB stick. This generally isn’t needed, but if you experience difficulties in performing a successful calibration you can forward the calibration log data to for further advice. By default, the calibration data logging is disabled.
- Página 22 5. If the measured values are within the required tolerances, the Passes Test icon will be If the measured values are within the required tolerances, the Passes Test icon will be displayed and a forwards arrow appears to allow the user to move on to measure the calibration displayed and a forwards arrow appears to allow the user to move on to measure the calibration solution.
- Página 23 12. The unit takes three readings at 260 and 330nm, 10. The Calibration Solution Measurement icon will at both 0.5mm and 0.2mm path lengths. At the end of be displayed for a second time. Open the instrument the readings, the unit will display the results of the lid and use a lint free cloth to clean the read heads.
- Página 24 19. The unit takes two readings at 260 and 330nm, at both 0.5mm and 0.2mm path lengths. At the Press the button adjacent to the Circular Arrow icon to repeat the 2nd calibration solution end of the readings, the unit will display the results of the measurements taken: measurement or press the button adjacent to the Back Arrow icon to return to the 1st calibration solution measurement screen.
- Página 25 21. The Calibration Solution Measurement icon will be displayed. Open the instrument lid and use a lint free cloth to clean the read heads. Open the instrument lid and pipette a 2.0μl aliquot of the calibration solution onto the lower read head. Close the instrument lid and press the key adjacent to the Tick icon...
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Página 26: Section 8 - Cleaning And Decontamination
SECTION 8 – CLEANING AND DECONTAMINATION CLEANING Wiping the sample from both the upper and lower read heads upon completion of each sample measurement with a lint free cloth is usually sufficient to prevent sample carryover and avoid residue buildup. Although generally not necessary, water aliquots can be used to clean the measurement surfaces after the measurement of particularly highly concentrated samples to ensure no residual sample is retained on either read head. -
Página 27: Section 9 - Accessories
Please also ensure you include a clear description of the fault and a completed copy of our Decontamination Certificate. Please clearly mark the package for the attention of the Service Department and post to the following address: Cole-Parmer Ltd. Beacon Road, Stone,... -
Página 28: Section 11 - Troubleshooting
SECTION 11 – TROUBLESHOOTING 11.1 CALIBRATION ERROR CODES If an error code is displayed during calibration it will be accompanied by a Clipboard icon and a number to indicate the cause of the error. The first time that an error is displayed, it will be possible to repeat the erroneous measurement. -
Página 29: Section 12 - Glossary Of Icons
SECTION 12 – GLOSSARY OF ICONS Error Code Symbol Issue Common Back key Common Tick icon - Done/yes Common Cross icon – Cancel/no Common Arrow icon – Move down, decrease Common Arrow icon – Move up, increase Common Arrow icon – Move forwards Common Arrow icon –... -
Página 30: Section 13 - Chemical Compatability
SECTION 13 – CHEMICAL COMPATABILITY Assay Chemical Concentration Sodium bicinchoninate Sodium carbonate Sodium tartrate 0.16%* Sodium hydroxide 0.1M* Sodium bicarbonate 0.95%* Copper (II) sulphate 0.08% Biuret Sodium potassium tartrate 0.9%* Biuret Copper (II) sulphate 0.3%* Biuret Potassium iodide 0.5%* Biuret Sodium hydroxide 0.08M Lowry... -
Página 31: Section 14 - Declaration Of Conformity
EU Declaration of Conformity Product Laboratory Equipment File Number P225 Manufacturer Authorised Cole-Parmer Ltd Cole-Parmer Representative Beacon Road Beacon Road Stone, Staffordshire Stone, Staffordshire ST15 0SA ST15 0SA United Kingdom... - Página 32 Thank you for reading this data sheet. For pricing or for further information, please contact us at our UK Office, using the details below. UK Office Keison Products, P.O. Box 2124, Chelmsford, Essex, CM1 3UP, England. Tel: +44 (0)330 088 0560 Fax: +44 (0)1245 808399 Email: sales@keison.co.uk...