Multi-Wavelength Mode - Cole-Parmer JENWAY Genova Nano Manual De Instrucciones

5. Lift the lid of the Genova Nano and pipette 2µl of water onto the read head. Close the lid.
Perform the blank reading.
6. Lift the lid and wipe the water from both the upper and lower read heads.
7. Pipette 2µl of the DNA sample onto the read head. Close the lid and perform the DNA reading.
Record the results.
6.3

Multi-wavelength Mode

This mode simply calculates the DNA concentration by multiplying by the factor 50 for dsDNA but
also allows correction by a reference wavelength at 320nm.
1. From the Life Science screen select the Multiwavelength mode:
2. Press the button next to the Settings Menu icon:
Wavelength 1: 260; Wavelength 2: 280; Wavelength 3: 230. Activate the reference Wavelength
4, 320nm. The units are µg/ml.
3. Touch the button next to the Calculation Factors icon:
Factor 2 = 0 and the Sum = (xF1*(A1-A4))-(xF2*(A2-A4)).
4. Press the button next to the Tick icon twice to confirm and return to the measurement screen.
5. Set the path length to 0.5mm. Press the button next to the Tick icon to confirm.
6. Lift the lid of the Genova Nano and pipette 2µl of water onto the read head. Close the lid.
Perform the blank reading.
7. Lift the lid and wipe the water from both the upper and lower read heads.
8. Pipette 2µl of the DNA sample onto the read head. Close the lid and perform the DNA reading.
Record the results.
18
.
. Check that the settings are as follows:
and check that Factor 1 = 50;