Dense And Viscous Liquids; Htl Pipette Tips; Recommendations - HTL LABMATE Soft Manual De Instrucciones

5 - DENSE AND VISCOUS LIQUIDS

The LAB MATE Soft specifications of accuracy and pre-
cision are based on pipetting distilled water. The han-
dling of liquids with physical qualities of density, viscosity
and surface tension differing extremely from water may
need a gravimetrically checked compensation of the vol-
ume setting. Normally the degree of error resulting from
heavy or viscous liquids is negligible if the pipetting is
done slowly and carefully. It is most important to give the
liquids some time to follow the change of pressure by
holding the pipette tip in its position for at least 2 sec.
after the aspiration and the blow out stroke.
If in extreme cases this method of operation does not
result in accurate values, a compensation could be
achieved as follows:
Weigh the liquid pipetted when the LAB MATE Soft is
set to the nominal value. Then calculate the set-off from
the nominal value:
Corr: val. = 2 x nom. val. -
- weight of the sample
m
γ - density of liquid
Check this operation once again and correct if neces-
sary. Note the corrected value for further pipetting the
same kind of liquid.

6 - HTL PIPETTE TIPS

HTL tips are made from high performance polypropylene
and their quality guarantees the precision and accuracy
associated with the LAB MATE Soft. Strict control is
maintained throughout the manufacturing process to
ensure the highest quality.
The accuracy and precision figures for the LAB MATE
Soft are only guaranteed when HTL tips are used. The
use of inferior quality tips will seriously degrade the per-
formance of the LAB MATE Soft.
Tips 10
These tips are used for volumes between 0.1 μl and 10 μl.
They are used with the LM2 and LM10 models, which
are equipped with a red pushbutton.
Tips 200
These tips are used for volumes between 2 μl and 200 μl.
They are used with the LM20, LM50, LM100 and LM200
models, which are equipped with a yellow pushbutton.
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m
-
γ
Tips 300
These tips are used for volumes between 50 μl and 250 μl.
They are used with the LM250 models, which are
equipped with a green pushbutton.
Tips 1000
These tips are used for volumes between 100 μl and
1000 μl. They are used with the LM1000 models, which
are equipped with a blue pushbutton.
Tips 5000
These tips are used for volumes between 1000 μl and
5000 μl. They are used with the LM5000 models, which
are equipped with a white pushbutton.
Tips 10000
These tips are used for volumes between 1000 and
10000 μl. They are used with the LM10000 models,
which are equipped with a white pushbutton.

7 - RECOMMENDATIONS

Observing the following recommendations will ensure
maximal possible accuracy and precision of liquid sam-
pling.
• Make sure to operate the LAB MATE Soft slowly and
smoothly.
• The depth of immersion in the sample liquid should be
the minimum necessary and should remain constant
during aspiration.
• The LAB MATE Soft should be held in a vertical posi-
tion.
• Change the tip when volume setting is changed or when
a different liquid is to be aspirated.
• Change the tip if a droplet remains on the end of the tip
from the previous pipetting operation.
• Each new tip should be pre-rinsed with the liquid to be
pipetted.
• Liquid should never enter the LAB MATE Soft shaft. To
prevent this:
- Press and release the pushbutton slowly and smoothly.
- Never turn the pipette upside down.
- Never lay the pipette on its side when there is liquid in
the tip.
• Never force the volume setting beyond its recommended
limits.
ENGLISH
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