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IMPORTANT!  Please
read these instructions
before operating your
Hoefer UVC5000 to
familiarize yourself with
operation procedures.

Introduction

®
The Hoefer
UVC5000 offers researchers an
instrument to quickly, safely and efficiently
expose samples to a controlled amount of
ultraviolet (UV) radiation. Exposure of samples
to UV provides for the following:
• Crosslinking of DNA or RNA to nitrocellulose,
nylon or reinforced nitrocellulose.
• PCR sample contamination control.
• Photonicking of DNA
• Testing RecA function
• Rapid site mapping
• UV sterilization and sanitization
• UV curing
The UVC5000 is designed to measure and
control the UV radiation within the exposure
chamber. A unique UV sensor continually
measures the UV energy and automatically
adjusts to variations in UV intensity that occur
as the UV tubes age. This same UV sensor feed-
back measurement system allows you to set UV
sample exposure, which automatically deacti-
vates the UV sources when the set UV energy
dose has been achieved.
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