Applications - Hoefer UVC5000 Manual Del Usuario

Applications

The Crosslinker is a multi-purpose UV exposure
instrument for use in the laboratory. A wide vari-
ety of applications for UV radiation exist in the
laboratory, including UV crosslinking of DNA
and RNA by covalently binding nucleic acids to
transfer membranes such as nitrocellulose, nylon
or nylon-reinforced nitrocellulose membranes
after Northern, Southern, slot or dot blotting.
The preset UV energy exposure setting is factory
set to a UV dose of 120,000 μJ/cm² for this
laboratory purpose. This setting has been found
to be the optimal dose for DNA retention and
hybridization-signal sensitivity.
Other applications:
• Nicking ethidium-bromide stained DNA in
agarose gels
• Gene mapping for creating cleavage-inhibiting
thymine dimers
• Testing RecA function
• UV sterilization
• Elimination of PCR contamination
• UV curing
• Fluorescence of materials
Further applications can be achieved with the
Crosslinkers by changing the UV wavelength
output of the UV lamps.
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