Optika Italy B-1000 Serie Manual De Instrucciones página 16

9.15 Use of oil immersion objective
1. Focus the specimen with a low power objective.
2. Lower the stage (remember to lock the coarse
upper limit knob).
3. Put a drop of oil (provided) on the area of the
specimen to be observed. (Fig. 28)
• Make sure that there are no oil bubbles. Air
bubbles in the oil damage the image quality.
• To check for bubbles: remove an eyepiece, fully
open the aperture diaphragm and observe the
objective exit pupil. (The pupil must be round and
bright).
• To remove the bubbles, gently move the nose-
piece to the right and to the left to move the im-
mersion objective a few times and allow the air
bubbles to move away.
4. Insert immersion objective in the light path.
5. Return the stage to the upper focusing point and
obtain an optimal focus using the fine focus knob.
6. After use, gently remove the oil with a soft pa-
per towel or a lightly moistened optic paper with a
mixture of ethyl ether (70%) and absolute alcohol
(30%).
• Immersion oil, if not immediately cleaned,
could crystallize creating a glass-like layer.
In this situation the observation of the speci-
men would be difficult if not impossible due to
the presence of an additional thickness on the
objective.
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F ig. 28 F ig. 28