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• the ambient temperature, and the temperature of the
pipettor, tips and water was identical
• the density of the liquid used was close to that of dis-
tilled water
• the balance with appropriate sensitivity has been used
Volume checked [μl]
0.1 - 10
10 - 100
> 100
• mg/μl conversion factor has been taken into account
If the above conditions are satisfied and the accuracy error
for selected volume given in the table, on the page 11
exceeds the permissible value, the pipettor recalibration
procedure should be carried out.
The recalibration can be performed within one full turn
of the key to the right or to the left only.
Recalibration conditions:
• Ambient temperature and the temperature of the pipet-
tor, tips and liquid should be within the range 20-25°C
and stabilized during weighing within ± 0.5°C
• Measurements should be conducted using distilled
water
• Balance sensitivity should be suitable for the volume to
be controlled.
Recalibration procedure:
• Set the dose volume depending on the pipettor volume
according to the following table:
Range
of the
Cat. No
Model
pipette
volumes
[μl]
613-2891
VWR2
0.1-2
613-2892
VWR10
0.5-10
613-2893
VWR20
2-20
613-2894 VWR100
10-100
613-2895 VWR200
20-200
613-2896 VWR1000 100-1000
613-2897 VWR5000 500-5000
613-2898 VWR10000 1000-10000
16
Balance sensitivity [mg]
≤
0.001
≤
0.01
≤
0.1
Volume
change DV
Preset
Permissible
for full turn
volume
volumes
of the cali-
[μl]
[μl]
bration key
[μl] (24
increments)
0.2
0.176-0.224
0.06
0.5
0.48-0.52
0.33
2
1.94-2.06
0.63
10
9.84-10.16
2.50
20
19.76-20.24
6.30
100
98.4-101.6
25.00
494 - 506
125.00
500
975-1025
250.00
1000
• Perform 5 aspirations, weigh each one and calculate
the average value of the aspirations
• Calculate average aspirated volume in μl multiplying the
average aspiration amount [mg] by the distilled water
density coefficient [μl/mg], which depends on tempera-
ture and pressure according to the following table:
Temperature [°C]
20
21
22
23
24
25
If the average aspirated volume exceeds the permissible
value, the following should be done:
• Take off the pipetting pushbutton, (Fig. 4A).
Warning: The pipetting pushbutton consists of 2 parts:
the knob (Fig. 1A2) and the pushbutton (Fig. 1A1). After
removal of pushbutton, both parts are separated.
• Holding the volume setting knob to protect it against
rotation, insert the calibration key into the cuts of the
calibration screw, (Fig. 4B)
• Turn the key clockwise to reduce the aspirated volume,
or counter-clockwise to increase the volume, (Fig. 4C).
One full turn of the calibration key changes the pipettor
aspiration volume by the amount given in the table
• Take out the key and fix the pipetting pushbutton,
(Fig. 4D). The pipetting pushbutton should be fixed in
opposite order.
Determine the average aspirated volume. The average
volume should be within the permissible range given in
the table. If the volume exceeds the values stated, the re
calibration procedure should be repeated.
Version: 1
Issued 17/03/2014
Pressure [kPa]
95.0
101.3
1.0028
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0035
1.0037
1.0038
1.0039
1.0040
ENGLISH
105.0
1.0029
1.0031
1.0033
1.0036
1.0038
1.0040
17
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