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LIMITATIONS
A negative test from a surface or liquid sample cannot exclude the possibility that the food contains
coconut residue since it may be distributed unevenly on the surface or rinse and may be below the
limit of detection of the test.
Reveal 3-D for Coconut Allergen is qualitative and should only be used as a preliminary screen for
the presence of coconut residue. The validity of results obtained should be viewed in conjunction
with data from a quantitative assay, such as Veratox for Coconut Allergen.
Hydrolyzed and fermented proteins may not be detectable with antibody-based test methods for
allergen testing. Although the proteins may be undetectable in the assay because of their properties,
there still could be allergenic protein residue present.
Validate detection by testing a positive control from the source of allergen or ingredient that is available
in your manufacturing environment and that may pose an allergen contamination risk to ensure the
test can detect the allergen of concern. Contact Neogen Technical Services for additional information.
Remove the following and allow to equilibrate at room temperature before use
(20–30 minutes out of refrigerator):
1.
1 sample tube and cap
2.
1 Type 8 extraction buffer sachet
3.
1 Reveal 3-D Coconut test device (in foil pouch)
1. Carefully tear/cut off the top of the Type 8 extraction buffer sachet and add
the entire contents to the sample tube.
2. Add 0.25 mL of sample to the sample tube. If a pipette is unavailable, 0.25
mL may be crudely estimated by half-filling a white sample tube cap.
NOTE: Test results will be less accurate.
3. Secure the white cap and shake for 1 minute.
Questions? Call Neogen at 800/234-5333 or 517/372-9200
Questions? Call 800/234-5333 or 517/372-9200
LIQUID SAMPLING
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