CORNING Stripettor Ultra Manual De Instrucciones página 7

Filling the pipet
Before aspirating is started, set the speed by pressing the SPEED switch (Figure 1C) until
the display shows the correct speed (Figure 1E).
• HIGH speed - fast aspirating (Figure 2.1),
• LOW speed - slow aspirating (Figure 2.2).
It is recommended to set the LOW speed when working with pipets of volumes up to 5 mL,
and the HIGH speed for pipets of volumes greater than 5 mL. Holding the pipet controller
in a vertical position, immerse the pipet end in the liquid to be drawn up (Figure 3B),
and press the aspiration button gently. The speed depends on how deep the aspirating
button has been pressed. The deeper the button is pressed the faster the liquid is aspirated
into the pipet.
It is recommended to draw a slightly greater liquid volume than required (due to meniscus
above the required volume mark), adjusting the aspiration speed, so as not to overfill the
pipet.
Setting the volume
After the pipet is filled, dry the outside surface with absorbent paper that does not leave
impurities. Then set the required liquid volume precisely. Pressing the dispense button gen-
tly (Figure 3C), dispense the excessive liquid from the pipet until the meniscus of the liquid
aligns exactly with the required volume mark on the pipet.
Emptying the pipet
Holding the vessel in an inclined position, place the pipet end in contact with the vessel wall
and press the dispense button gently (Figure 3C). The dispensing intensity may be adjusted
depending on how deep the dispense button has been pressed. The deeper the button is
pressed the faster the outflow of liquid from the pipet.
The pipet controller has two dispense modes. The dispense mode is selected by successive
pressing the MODE switch (Figure 1D) until the display shows the correct mode (Figure 1E).
• Gravity mode marked with the
in gravity mode, which means that the liquid flows out of the pipet by its own weight.
• Blow out mode marked with the
in gravity mode, however, when the dispense button is pressed to the middle position,
the pump is started and fast emptying of the pipet with a blow out is effected.
CAUTION:
During gravimetrical dispensing the pipet is not completely emptied due to the characte -
ristics of pipets used with the pipet controller.
icon on the display (Figure 2.4) – dispensing is effected
icon on the display (Figure 2.5) – dispensing is effected
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