Chemical Integrators; Biological Indicators - Tecno-gaz ONYX B 7.0 Instrucciones De Uso

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13.1 Chemical integrators

These tests (spare 0code 200/S and 215-S) are based on the properties of indicator dyes
that are able to modify their own aspect if they are adequately exposed to heat according
to given times, since they interact with all the cycle parameters (temperature and time). If
the test is successfully completed, the correct stability is validated.
USE
RESPONSE
PERIODICITY

13.2 Biological indicators

The test (spare code TS002ZBK) is used to prove the autoclave capability as to the
complete destruction of all microorganisms. It is represented by a standardized spore
preparation having biological characteristics, high resistance to heat, which is to be
deemed an absolutely safe control tool (ATCC 7953).
It consists of spore ampoules, it is non pathogenic, non toxic and non pyrogenic.
USE
RESPONSE
AUTOCLAVE VALIDATION TESTS
The chemical integrators (spare code 200/S o 215-S) are to be put into the
sterilization chamber before starting the cycle, even with the load already
in. The integrators can either be unwrapped and positioned into the trays
or they can be wrapped into the sterilization bags together with the
materials to be sterilised. In any case it is advisable to wrap the integrators
where there are many operators. As a matter of fact by doing so the
sterilization of every single tool will be certified.
There is no preset testing interval, apart from areas to which specific
regional regulations apply. In any case all the chemical integrators are the
cheapest and quickest available tests, hence in order to constantly monitor
the autoclave performance, it is advisable to use them on all of the
cycles or at least once a day.
The ampoules shall have to be put into the sterilization chamber even
when loaded.
Carry out the cycle and then take out the ampoule by taking care since it is
hot and pressurized.
Leave it cool down for about 10 minutes and then activate it by taking care
so as to keep the ampoule always upright. The chemical indicator placed
on the label of the ampoule will have changed its colour from blue into
black. Subsequently incubate the ampoule into a biological incubator,
together with an unprocessed test ampoule, that has anyway be activated.
After 48 hours take the processed ampoule out of the incubator and
assess the test result. If the ampoule has changed its colour to yellow this
means that the autoclave has failed the test and there is bacterial growth.
If the ampoule remains purple, this means that there is no bacterial growth
and hence the test succeeded. The test ampoule of course will always
ENGLISH
If the colour change remains
within the white area, the test
failed
If the colour change enters the
SAFE window, the test
succeded
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