Adjusting Transmitted-Light Brightfield On The Full Köhler Microscope - Zeiss Primo Star Instrucciones De Manejo

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• Turn the focusing ring of the eyepiece (Fig. 7/3) to focus on the triangular figure of the eyepiece
pointer.
• Put the specimen onto the mechanical stage. Look at the specimen through the eyepiece with the
eyepiece pointer and focus on the microscopic image by turning on the focusing drive.
• When in the above-mentioned eyepiece both microscopic image and eyepiece pointer appear sharply,
focus the image for the second eye by turning the focusing ring of the second eyepiece.
Having done so, both microscopic images inclusive of the eyepiece pointer are focused.
Afterwards, you should focus on the specimen only by adjusting the focusing drive.
3.3.3
Adjusting transmitted-light
brightfield on the Full Köhler
microscope
• First, place a contrasty specimen slide with the
0.17 mm cover slip being on top in the
specimen holder of the mechanical stage. Fix
the slide by means of the spring lever
(Fig. 4/12).
• If the microscope stand is equipped with a
phase or dark-filed slider, pull this slider out to
the left as far as it will go.
• Turn rotary knob (Fig. 8/1) on microscope stand
to adjust the illumination intensity.
• Move the Abbe condenser up to the top
mechanical stop by turning knurled knob
(Fig. 4/15); set the control lever of the aperture
diaphragm (Fig. 4/14) to mid-position.
The
knurled
adjustment of the condenser is at the
left of the microscope, if you use the
mechanical stage 75x30 with drive on
the right, it is at the right, if the stage
controls are on the left.
• Turning at the knurled ring (Fig. 8/6) of the
nosepiece, swing the 10x objective into the
light path.
• On the binocular tube (Fig. 8/7), first look
through one eyepiece and turn the focusing
drive (Fig. 8/2) to focus on the specimen.
• Then, readjust the focus for the other eye, if necessary, by turning the eye lens of the focusing
eyepiece.
• Close the luminous-field diaphragm (Fig. 8/3) until it becomes visible (even if not in focus) in the field
of view (Fig. 8/A).
B 46-0071 v 03/06
Start-Up and Operation
knob
for
vertical
Fig. 8
Adjusting transmitted-light
brightfield
Carl Zeiss
17

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