Gel Casting; General Rules - VWR shiroGEL Vertical 20 Manual De Instrucciones

Gel Casting for 2 Gels Using the shiroGEL VERTICAL 20

General rules

1. Clean a set of glass plates for each gel first with distilled water and then with 70
% ethanol. One set of glass plates constitutes one notched glass plate and one
plain glass plate with bonded spacers. When using a 2-gel glass plate sandwich
in the shiroGEL VERTICAL 20 to cast 2 gels either side of the PAGE insert, two
notched glass plates are required - one with and the other without bonded
spacers - as well as a set of plain glass plates with bonded spacers (Please also
see section on Converting the shiroGEL VERTICAL 20 from a 2- to 4-gel
Configuration). The plain glass plate is positioned outermost, followed by a
notched glass plate with bonded spacers and then a second notched glass plate
(Please see Fig. 2. Glass plate set-up for 2-gel sandwich using yellow
spacers).
N.B. - All glass plates, modules and casting base accessories must be
completely dry during set – up. Wet components are more likely to misalign
and cause leaks.
2. Assemble the glass plates so that the bottom of the glass plates and the spacers
are perfectly aligned; the standard glass plates supplied with the shiroGEL
VERTICAL 20 or shiroGEL VERTICAL 20 systems, are supplied with bonded
spacers and do not require manual alignment. However, if using free spacers,
which are not included as standard, these need to be perfectly aligned. This is
best performed using a small spacer or comb to push the spacers apart.
NOTE: The glass plates with bonded spacers have an arrow in the top of
the spacers which are slightly longer than the glass plate to indicate the
top.
10