Use Of The Microscope (B-510Fl); Use Of The Shutter - Optika Italy B-510 Serie Manual De Instrucciones

14.2 Use of the microscope (B-510FL)

1. Turn on the power supply ① for the mercury bulb
and wait 5 minutes for the arc to stabilize. (Fig.
67).
2. Move the filter selector ② to one of the four
available positions until the click stop. (Fig. 68).
• The microscope has a 4-position filter holder
slider. The positions 1 and 2 are empty to house
additional filters, position 3 houses a B filter and
position 4 a G filter.
FILTER EXCITATION
CUBE FILTER
B 460-490 nm
G 510-550 nm

14.2.1 Use of the shutter

• The microscope is equipped with a shutter
③ located on the right side of the fluorescent
illuminator. (Fig. 69)
1. Close the shutter by interrupting the observation
for a limited time and not subjecting the sample
to unnecessary lighting in the period in which it
is not observed. (Switching off and switching on
frequently the HBO lamp considerably reduces its
duration).
• This precaution is not necessary in the case
of the LD1 and LD2 models: the LED can be
switched on and off without any problem.
DICHROIC EMISSION
MIRROR FILTER
505 nm 515LP nm
570 nm 575LP nm
• FITC: fluorescent antibodies
• Achridine orange: DNA, RNA
• Auramine
• Rhodamine, TRITC: fluorescent antibodies
• Propidium iodide: DNA, RNA
• RFP
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②
APPLICATIONS
③
①
Fig. 67
Fig. 68
Fig. 69